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API
| Question | Answer |
|---|---|
| What would the results for pathogenic Neisseria meningitidis on the API Quad Ferm look like? | It would be positive for glucose and maltose (yellow) and negative for lactose and sucrose (blue). |
| What should you do if the glucose is negative on the API? | Check to see if 3 tests are positive - if not, set up OF dextrose and motility test and reincubate for an additional 24 hours. |
| After reincubation of an API strip with a negative glucose, what additional tests will you then read that are NOT read for glucose fermenters? | Oxidase and nitrate; also OF dextrose and motility. |
| What API wells require an anaerobic environment? How is this achieved? | The H2S, urease, and decarboxylase (ADH, LDC, ODC) wells. By overlaying them with oil. Note: oil is also important for urease to contain the byproduct (ammonia). |
| How should the API strips be incubated and why is it important to do it this way? | At 35 degrees in a non-CO2 incubator - CO2 can interfere with the pH indicators in the strips. |
| What is a pitfall with using the API strips instead of MIC/ID? | With the MIC/ID panel, you get antibiotic susceptibility results in the same place. With API, you must use another method to get these results. They must be read manually, whereas MIC/ID can be read by the machine. |
| What is a pitfall with using the API strips instead of Maldi Tof or Vitek? | It takes much longer to get results. Maldi Tof reads results in just minutes, but the API must be incubated for 24 hours and must be read manually. |
| What is the benefit to using the API strips over Maldi Tof or Vitek? | They are read manually, but if the machine breaks in these other systems, results cannot be read. |
| What should be incubated at the same time as the strip? | A purity plate, so that one can ensure the culture was pure and results are accurate. |
| How is the API strip interpreted? | The technologist decides which tests are positive or negative, and adds up the corresponding numbers to come up with a 7 digit profile number to look up in the API system for identification. |
| What organisms is the API strip particularly good for identifying? | Bacteria from the family Enterobacteriaceae and other non-fastidious gram negative rods. |
Created by:
laceymaej
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