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Unit 2

Normal Mouth Flora/Presumptive N. gonorrhoeae

What are the two pathogenic mouth flora species? Streptococcus pyogenes and Arcanobacterium haemolyticum.
Which test employs the fermentation of four different sugars to differentiate pathogenic Neisseria from saprophytic Neisseria? API Quad-Ferm+
True or false: Staph aureus, Micrococcus, and yeast are all considered normal oral flora? True. Normal Gram positive oral flora include Alpha strep, Staph epidermidis, Staph aureus, Strep pneumo, Beta Strep Groups B and F, small colony morphology Beta Strep Groups C and G, Gamma Strep, Diphtheroids, Micrococcus, and Aerococcus.
What antibiotics are present in Modified-Thayer-Martin media and what are their functions? Vancomycin to inhibit Gram positive organisms, Colistin to inhibit Gram negative rods, Nystatin to inhibit yeasts, and Trimethoprim Lactate to inhibit Proteus and Gram negatives that are resistant to Colistin.
What does Arcanobacterium haemolyticum look like on Gram stain and on agar? Arcanobacterium haemolyticum looks like a Diphtheroid-like Gram positive rod on Gram stain and resembles Beta strep on agar.
What are the normal Gram negative mouth flora species? Gram negative rods, saprophytic Neisseria species, Haemophilus parainfluenzae, and Haemophilus influenzae.
How does pathogenic Neisseria appear on Gram stain? Gram negative diplococci
What locations must a presumptive Neisseria gonorrhea sample be cultured from? Normal sexual sites including the vagina or urethra.
What type of test is the Gonogen II and what does it test for? It is a monoclonal antibody test that is used to confirm Neisseria gonorrhea.
What species is routinely looked for on all throat cultures from patients less than 30 years old? Arcanobacterium haemolyticum is routinely tested for on all throat cultures from patients under 30 as it can cause severe pharyngitis.
Created by: nkniep



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