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L32: Reg Gene Expr

USCSOM: Biochemistry: L32 Regulation of Gene Expression

QuestionAnswer
Describe lactose. galactose linked Beta-1-4 glucose
What two ways does lactose enter E. coli? lactose permease and hydrolytic enzyme that splits lactose into galactose and glucose
What does coordinate regulation mean? The activity of both genes are coupled to each other
Explain catabolite repression in the lac E. coli pathway activity of lactose enzyme is decreased when glucose is also added
What is the order of the polycistronic messenger for lactose metabolism in E. coli? Promoter - lacZ - lacY
What does lacZ gene promote? Beta-galactosidase
What does the lacY gene promote? lactose permease
What is an operon? a set of genes that are under control of a single operator
What happens when an inducer binds to a repressor? repressor loses its affinity and opens up promotor for RNA polymerase
What is CRP/CAP? cAMP regulator protein/ cAMP activator protein; binds upstream of promotor
What two things are necessary to get good expression of the lac operon? inducer present; CRP bound
What increases CRP's affinity to bind to DNA? cAMP
How to cAMP levels vary with respect to the growth rate in E. coli? inverse
Catabolic pathways are controlled by induction or repression? induction by substrate
Anabolic pathways are controlled by induction or repression? repression by products
What happens to activity in the lac operon in AAs only?
What happens to activity in the lac operon in AA plus lactose?
What happens to activity in the lac operon in AA, plus lactose, plus glucose?
What happens to activity in the lac operon when mutation inactivates the repressor?
What happens to activity in the lac operon when mutation allows repressor to bind to DNA but lose affinity for allolactose?
What happens to activity in the lac operon when mutation lies in the operator such that the repressor no longer binds to it?
What happens to activity in the lac operon when mutation inactivates the CRP protein?
What happens to activity in the lac operon when mutation interferes with synthesis of cAMP
Created by: jlellerm
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