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Microbiology

Aerobic Gram-Positive Cocci

QuestionAnswer
A urine Gram stain shows gram-positive cocci in clusters. The organisms tested catalase positive. To SPECIATE this organism form culture, the tech should perform a coagulase test and a/an: novobiocin susceptibility
The Gram stain from a blood culture shows gram-pos cocci in chains. No growth occurs on blood agar plates incubated aerobically and anaerobically. Additional testing should be done to detect the presence of: Abiotrophia defectiva
Viridans streptococci can be differentiated from Streptococcus pneumoniae by: bile solubility
A reliable test for distinguishing Staphylococcus aureus from other staphylococcus is: coagulase
The optochin (ethylhydrocupreine hydrochloride) disk is used for the identification of: Streptococcus pneumoniae
In the optochin susceptibility test, if there is a zone of inhibition of 19-30mm surrounding the disk following overnight incubation at 37C, the colony MOST likely consist of: pneumococci
Which 2 diseases are usually preceded by infection with beta-hemolytic streptococci? glomerulonephritis and rheumatic fever
The enterotoxin produced by certain strains of hemolytic, coagulase positive Staphylococcus aureus: causes one type of bacterial food poisoning
A gamma-hemolytic Streptococcus that blackens bile esculin agar but does not grow in 6.5% NaCl broth is MOST likely: group D Streptococcus
Gram stain examination from a blog culture bottle shows dark blue, spherical organisms in clusters. Growth on sheep blood agar shows small, round, pale yellow colonies. Further tests should include: catalase production and coagulase test
Gram-positive cocci in chains are seen on a Gram stain from a blood culture. The organism grows as a beta-hemolytic colony. Further tests that could be performed include: bile esculin, PYR, bacitracin, and hippurate
"Nutritionally deficient" streptococci are: in the genera Granulicatella and Abiotrophia
A beta-hemolytic streptococcus that has been isolated from an ear culture grows up to the edge of a 0.04 unit bacitracin disk. Which tests would help to determine if the organism is Entercoccus? growth in 6.5% NaCl broth
The organism MOST commonly associated with neonatal purulent meningitis is: group B streptococci
An important cause of acute exudative pharyngitis is: Streptococcus pyogenes
Which is the MOST frequent cause of prosthetic heart valve infections occurring within 2-3 months after surgery is: Staphylococcus epidermidis
Which of the 2 different antimicrobial agents are commonly used and may results in synergistic action in the treatment of endocarditis caused by Enterococcus faecalis? a penicillin derivative and an aminoglycoside
Children who have infections with beta-hemolytic streptococci can develop: acute glomerulonephritis
A gram-pos coccus isolated from a blood culture has the following characteristics: optochin suscept-neg, bacitracin suscept-neg, bile esculin hydrolysis-neg, hippurate hydrolysis-pos, catalase-neg. The organism is MOST likely: Streptococcus agalactiae
A beta-hemolytic streptococci that is bacitracin-sensitive and CAMP-negative is: group A
A beta-hemolytic Streptococcus that is bacitracin-resistant and CAMP-positive is" group B
Group B, beta-hemolytic streptococci may be distinguished from other hemolytic streptococci by which procedure? latex antigen typing
It is important to differentiate between Enterococcus and group D strep because: enterococci often show more antibiotic resistance than group D streptococci
Streptococcus pneumonia can be differentiated best from the viridans group of streptococci by: bile solubility
Characteristically, enterococci are: relatively resistant to penicillin
A beta-hemolytic, catalase positive, gram-positive coccus is coagulase negative by the slide coagulase test. Which is the MOST appropriate action in identification of this organism? do a tube coagulase test to confirm the slide test
Which would best differentiate Streptococcus agalactiae from Streptococcus pyogenes? hydrolysis of sodium hippurate
The MOST critical distinction between Staphylococcus aureus and other Staphylococcus is: coagulase production
Which organisms is, to date, considered universally susceptible to penicillin: Streptococcus pyogenes
A beta-hemolytic gram-pos coccus was isolated from CSF of a 2 day old infant w/signs of meningitis. This isolate grew on sheep blood agar under aerobic conditions and was resistant to a bacitracin disc. What should be performed for the identification? latex antigen testing
How many hours after eating contaminated food do initial symptoms of staphylococcal food poisoning typically occur? 2-6 hours
During the past month, Staphylococcus epidermis has been isolated from blood cultures at 2-3 times the rate from the previous year. The MOST logical explanation for the increase in these isolates is: there has been a break in proper skin preparation before drawing blood for culture
An outbreak of Staphylococcus aureus has occurred in a hospital nursery. In order to establish the epidemiological source of the outbreak, the MOST commonly used typing method is: pulsed-field ges electrophoresis
A yellow colony from a wound culture tested catalase-positive and coagulase-negative. The organism stained as gram-positive cocci in clusters. Which tests would differentiate between coat-neg staph and Micrococcus? furazolidone susceptibility
A light yellow colony from a skin lesion grew aerobically and was catalase positive and coag neg. Gram stained as positive cocci in clusters, modified oxidase positive, bacitracin susceptible and resistant to lysostaphin. The identification is? Micrococcus luteus
An isolate of unknown beta-hemolytic Strep is streaked perpendicular to a streak of beta-lysin producing S. aureus. Zone of arrowhead hemolysis is noted. What is the name of the test and presumptive ID of unknown strep? CAMP test and S. agalactiae
Which organism may be used as a positive quality control organism for the bile esculin test? Enterococcus faecalis
Created by: emery87