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Cytogenetic:Overview
AGT Lab Manual Information
| Where to find spontaneously dividing cells | bone marrow, lymph node, testis, chorionic villi of placenta, some leukemic blood, solid tumor, some pleural or ascetic fluid, fetal ascites or cystic hygroma, and often fetal or newborn blood; plus blood lymphocytes, amniotic fluid, skin and fibroblasts |
| Mitotic arresting agents | Colcemid, colchicine, or Velban |
| Who coined the words mitosis, chromatin, prophase, metaphase, anaphase, and telophase | Walther Flemming, 1879-1889 |
| Who coined the word chromosome in 1888 | W. Waldeyer |
| Q banding method discoverer | Caspersson in 1968: quinacrine mustard stain; allowed identification of each pair as well as small parts of each chromosome |
| Tissue mosaic syndromes | Pallister-Killian syndrome and trisomy 20 mosaicism |
| Suspension Culture tissue types | bone marrow, blood, some neuroblastomas, and most lymphomas, suspended in growth medium and do not attach to the culture vessel |
| Attached monolayer culture tissue types | amniocytes, skin fibroblasts, which require adherence to the culture vessel to grow |
| Fanconi's anemia open system gas exchange | no oxygen and may be gassed only with nitrogen (sensitive to the presence of oxygen) |
| Media with buffering systems | CO2-bicarbonate equilibrium and HEPES buffer |
| RPMI 1640 type of culture | Blood, bone marrow, solid tumor |
| RPMI 1603 type of culture | Blood, bone marrow |
| Media for all types of culture | MEM or Eagle's MEM (minimal essential medium); MEM Alpha; DME (Dulbecco's modified Eagle's); Ham's F10; Ham's F12; McCoy's 5A |
| Fragile X media | Medium 199 or TC 199 (low folic acid media) |
| Media for Amnio and CVS | Chang; Amniomax; Amniogrow (all for neoplastic cells) |
| Relative centrifugal force calculation | RCF = 0.00001118 x r(radius in cm) x Nsquared (rotating speed, RPM) |
| Hypotonic affects: | spreading, chromatid width and separation, and in bloods and bone marrows, the elimination of red blood cells |
| Colcemid concentration affects: | chromosome spreading and morphology |
| Increased dosage of Colcemid: | increases MI, straightens chromosomes, crisps chromatid edges, and increases chromosome spreading as it releases them from the mitotic apparatus |
| colchicine, a derivative of | autumn crocus |
| Velban | Vinblastine sulfate, mitotic arrestant |
Created by:
MorganRiso
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