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UND 363 Rhodanin Cu
UND 363 Rhodanine Copper
| Question | Answer |
|---|---|
| purpose for rhodanine method | id of copper in tissue (ie liver for wilsons disease) |
| principle | rhodanine demo's protein that copper binds to not the copper itself. |
| what is the difference between rhodanine method and the rubeanic acid methods | rhodanine is more sensitive, but is less specific and may lead to false positives |
| fix for rhodanine method | NBF |
| tech | 6-8 (6-10 per sheehan and thicker will stain better) |
| qc | section with copper |
| reagents | SATURATED rhodanine working - binds copper, mayers hematox - stain nuclei, sodium borate - bluing agent |
| results | copper - bright red to red yellow, nuclei - lt blue |
| what will occur if copper conc. is low | fading may occur after coverslipping (therefore copper may be difficult to distinguish from lipofuscin) |
| what can stop the inherent fading if copper conc. is low | use apathy medium for mount |
| what can also be used as a control | fetal liver (in NBF), fixed no longer than 24 hrs berfore paraffin processing. |
| what occurs if overstaining is done with hematox | copper will be masked |
| what type of reagents should be used | analytical grade |
| since the rhodanine method detects protiens that bind Cu what can occur | false positives |
Created by:
mustangvxd
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