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UND 363 Alizarin Red

UND 363 Alizarin Red S Calcium

QuestionAnswer
purpose for Alizarin Red S id of calcium in tissue
principle calcium forms an alizarin complex via a chelation process
fix alcoholic formalin or NBF (acidic fixes and pretreatments must be avoided)
tech 4-5 and FS
qc section with calcium
reagents alizarin red s/ammonioum hydroxide - chelation (ph must be 4.1-4.3 CRITICAL), acetone - dehydration, acetone/xylene - dehydration
what is used to adjust the ph of this stain .5% ammoniom hydroxide
results calcium - orange red
what are 2 importants facts about this stain reaction product is birefringent, and the reaction must be controlled microscopically to prevent diffusion artifact
what does the dahl version of this use in addition to the reagents lt green counterstain
Created by: mustangvxd