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UND 363 Bile (hall)

UND 363 Bile stain (Hall)

QuestionAnswer
purpose for Hall Bile stain demo presence of bilirubin in tissue and distinguish bile pigments (as a result of red cell breakdown)
principle for Hall Bile stain oxidation of bilirubin to biliverdin in acid medium (turns green). oxidation done by ferrich chloride in trichloroacetic acid medium (AKA FOUCHETS REAGENT)
fix for Hall Bile stain NBF
tech 4-5 and FS
qc tissue with bile (eg obstructed bile duct)
reagents for Hall Bile stain fouchets (ferric chloride 10% and trichloracetic acid) - oxidation, acid fuchsin/picric acid (van gieson) - counterstain
results bile/bilirubin - green (emerald - olive), background - yellow
Created by: mustangvxd
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