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UND 363 Gomor urates

UND 363 Gomori Methanimine silver urates

QuestionAnswer
purpose for Gomori Methenamine silver demo urates. unstable uric acid metabolism (gout) caused deposits of urate crystals around joints and in soft tissue
what are the collections of urate crystals called gouty tophi
principle for Gomori Methenamine silver urates stained black (presumed via silver reaction) and then reduced to metallic form
fix ABSOLUTE ALCOHOL REQUIRED
tech 4-5 and chem cleaned glass
qc for Gomori Methenamine silver section with urates
reagents silver nitrate/hexamethylenetetramine/sodium borate - impregnation, sodium thiosulfate - remove unreduced silver, lt green - counterstain
results urates - black, background - blue green
what can also be demoe inadvertantly with this tech. calcium, if the deposites are large enough to withstand dissolving in silver stain soln'
Created by: mustangvxd