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UND 363 Grimelius

QuestionAnswer
purpose for Grimelius stain demo argyrophil granules in neurosecretory tumors
principle for Grimelius stain argyrophil tech. therefore can bind silver but cannot reduce w/t reducer substance
fix for Grimelius stain NBF, but Bouins give a better intense stain and result
tech 4-5 chem cleaned glass
qc argyophil pos. (carcinoid tumor preferred), but can used sm. intestine
reagents silver nitrate/acetic acid/sodium trihydrate - impregnation, hydroquinone/soduim sulfite - reducig agent, N fast red - counterstain
results argentaffin/argyrophil granules - dk brown to black, nuclei - red, background - pale yellowbrown
since argentaffin and argyrophil substances stain what should be done to differentiate them use both grimelius and fontana masson (with 2 diff slides) and compare
Created by: mustangvxd
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