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UND 363 Schmorl Ferr

UND 363 Schmorl Ferric reduction

QuestionAnswer
purpose for Schmorl Tech id reducing substances (will stain melanin, argentaffin granules, and formalin pigment)
principle for Schmorl Tech reducing substances reduce ferric ions to ferrous ions, ferrous ions combine with ferricanyide present in stan to form insoluble precipitate of ferrous ferricyanide (turnbull blue)
fix for Schmorl Tech NBF preferred
tech 4-5 chem cleaned glassware
qc for Schmorl Tech section with melanin or argentaffin granules
reagents ferric chloride/potassium ferricyanide "working mix" - reduce ferric ions to ferrous ions that react with ferricyanide to form ferrous ferricyanide, mucicarmine - stain's mucin, metanil yellow - counterstain
what should the ferric chloride potassium ferricyanide working solution be adjusted to and with ph of 2.4 with 1N HCL
what will the metanil yellow and mucicarmine do to the turnbull blue color cause it to turn green
results for Schmorl Tech reducing substances - bluegreen, goblet cells/mucin - rose, background - yellow green
what has the schmorl tech been replace by IHC
what can be used instead as a counterstain nuclear fast red
if solutions are not fresh what will occur increased undesirable background staining
what does this tech work we with GI tract - for the demonstration of mucin
Created by: mustangvxd
Popular Histology sets

 

 



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