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UND 363Turnbull Blue

UND 363 Turnbull Blue

purpose for Turnbull Blue demo FERROUS (Fe2+) iron
why is ferrous iron not normally stored in tissue it is toxic, if it absorbed by intestine but is rapidly converted to ferric form
principle for Turnbull Blue sections treated with acidic potassium FERRICcyanide, ferrous iron will form insoluble bright blue pigment (turnbull blue ie ferrous ferricyanide)
fix for Turnbull Blue alcohol or NBF
techfor Turnbull Blue 4-5 and chem cleaned glass
qc section with FERROUS iron
reagents in order hcl/potassium FERRICcyanide mix -ferrous ions react to form ferrous ferrocyanide, acetic acid - rinse, nuclear fast red - counterstain
results ferrous iron - blue, background - pink/red
Created by: mustangvxd