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UND 363 Prussian blu

UND 363 Prussian Blue

QuestionAnswer
purpose for Prussian Blue (Lillie) detection of FERRIC iron (Fe3+)
where is ferric iron normally found in small amount in bone marrow and spleen
when too much iron is stored what is the condition called idiopathi memochromatosis (or hemosiderosis)
principle for Prussian Blue (Lillie) detect FERRIC ion in loosely bound protein (and hemoglobin, hemosiderin, myoglovin) complexes, treament w/ acidic solution of potassium FERROcyanide - any free ferric iron will form insoluble bright blue pigment
since the principle for Prussian Blue (Lillie) uses an acidic solution of potassium ferrocyanide how can the stain sensitivity be affected influenced by heat, ph, stain time, and amt of iron retained in tissue
fix for Prussian Blue (Lillie) alcohol or NBF (no acidic fix or chromate due to interferance of iron preservation)
tech for Prussian Blue (Lillie) 4-5 micron and chem. cleaned glassware
qc for Prussian Blue (Lillie) section with ferric iron. Excessive amts. not desirable due to reaction product being slightly soluble and can contaminate solution and give background stain
what is an alternative for qc sections for Prussian Blue (Lillie) post morte lung w/ iron and macrophages (aka hear failure cells)
reagents for for Prussian Blue (Lillie) potassium ferrocyanide/hcl mix - ferric ions react to form ferric ferrocyanide, nuclear fast red - counterstain
results for Prussian Blue (Lillie) nuclei and hemofuchsin - bright red, hemosiderin(iron) blue, background - pink
what will inadequately clean glassware (previous iron solutions) cause diffuse background staining
how can iron be dissolved and what should be done for bone bx iron can be dissolved by acidic fixes, but iron can still be demo'd in bone bx if fixed overnight in zenkers with a 3% acetic acid soln'
what should be used instead of zenkers for decal (due to mercury content) fixative with acetic or formic acid
what will occur if the ferrocyanide is heated will produce fine granular blue deposit (best done at room temp)
with all the solutions what is important to remember they do not contain iron (even the H20)
what will occur if the Nuclear fast red is not sufficiently removed with wter before dehydration cloudy slides will result, can be redone by returning to water (wash well) and then rehydrate and clear
Created by: mustangvxd
 

 



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