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UND 363 Prussian blu
UND 363 Prussian Blue
| Question | Answer |
|---|---|
| purpose for Prussian Blue (Lillie) | detection of FERRIC iron (Fe3+) |
| where is ferric iron normally found | in small amount in bone marrow and spleen |
| when too much iron is stored what is the condition called | idiopathi memochromatosis (or hemosiderosis) |
| principle for Prussian Blue (Lillie) | detect FERRIC ion in loosely bound protein (and hemoglobin, hemosiderin, myoglovin) complexes, treament w/ acidic solution of potassium FERROcyanide - any free ferric iron will form insoluble bright blue pigment |
| since the principle for Prussian Blue (Lillie) uses an acidic solution of potassium ferrocyanide how can the stain sensitivity be affected | influenced by heat, ph, stain time, and amt of iron retained in tissue |
| fix for Prussian Blue (Lillie) | alcohol or NBF (no acidic fix or chromate due to interferance of iron preservation) |
| tech for Prussian Blue (Lillie) | 4-5 micron and chem. cleaned glassware |
| qc for Prussian Blue (Lillie) | section with ferric iron. Excessive amts. not desirable due to reaction product being slightly soluble and can contaminate solution and give background stain |
| what is an alternative for qc sections for Prussian Blue (Lillie) | post morte lung w/ iron and macrophages (aka hear failure cells) |
| reagents for for Prussian Blue (Lillie) | potassium ferrocyanide/hcl mix - ferric ions react to form ferric ferrocyanide, nuclear fast red - counterstain |
| results for Prussian Blue (Lillie) | nuclei and hemofuchsin - bright red, hemosiderin(iron) blue, background - pink |
| what will inadequately clean glassware (previous iron solutions) cause | diffuse background staining |
| how can iron be dissolved and what should be done for bone bx | iron can be dissolved by acidic fixes, but iron can still be demo'd in bone bx if fixed overnight in zenkers with a 3% acetic acid soln' |
| what should be used instead of zenkers for decal (due to mercury content) | fixative with acetic or formic acid |
| what will occur if the ferrocyanide is heated | will produce fine granular blue deposit (best done at room temp) |
| with all the solutions what is important to remember they do not contain | iron (even the H20) |
| what will occur if the Nuclear fast red is not sufficiently removed with wter before dehydration | cloudy slides will result, can be redone by returning to water (wash well) and then rehydrate and clear |
Created by:
mustangvxd
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