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UND 363 Alc Blu 2.5

UND 363 Alcian Blue 2.5

purpose for Alcian Blue 2.5 demo mucoid capsule of cryptococcus
principle for Alcian Blue 2.5 (cryptococcus) alcian blue color due to copper, used in 3% acetic acid A.B stains sulfated and carboxylated acid mucopolysaccharides. A.B forms salt linkages with acid groups of acid mucopolysaccharides
fix for Alcian Blue 2.5 (cryptococcus) NBF or bouins
micron for Alcian Blue 2.5 (cryptococcus) 4-5
Qc section with know cryptococcus
reagents in order for Alcian Blue 2.5 (cryptococcus) pre 3% acetic acid - protect PH, alcian blue - stain mucin, post 3% acetic acid - prevents nonspecific staining, nuclear fast red - counterstain
results cryptococcus - pale blue, background - pink to red
Created by: mustangvxd