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UND 363 Mucicarmine

purpose for Mucicarmine demo cryptococcus mucoid capsule
Principle for Mucicarmine (cryptococcus) aluminum forms chelation complex with carmine which results in a net positive charge with attaches to acid groups of mucin
fix NBF
micron 4-5 or FS 6 micron
Qc slide with cryptcoccus
Reagents in order weigerts iron heme (soln' A&B) - nuclear stain, metanil yellow - counterstain, mucicarmine stain - stain mucin deep rose
what are the A and B solutions of Weigerts comprised of soln' A - hematox and alcohol, soln' B - HCL/ferric chloride/water
what makes up the mucicarmine stain carmin/alum lake, aluminum hydroxide (forms the chelation complex), ethyl alcohol (50 and 25%), anhydrous aluminum chloride
results for Mucicarmine (cryptococcus) mucin - deep rose to red, capsule of cryptococcus - deep rose to red, nuclei - black, other tissue - blue or yellow
K.H. explaination of the principle muci w/ aluminum mordant stains mucin to deep rose red. Aluminum is believed to for chelation agent binding acid groups of mucin and attaching red color to complex in a dye lake formation
what can obscure carminophilic properties overstaining with hematoxylin or metanil yellow
what precaution must be used with anhydrous aluminum chloride use under fume hood due to hydrogen chloride vapors
Created by: mustangvxd



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