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UND 363 Mucicarmine
Question | Answer |
---|---|
purpose for Mucicarmine | demo cryptococcus mucoid capsule |
Principle for Mucicarmine (cryptococcus) | aluminum forms chelation complex with carmine which results in a net positive charge with attaches to acid groups of mucin |
fix | NBF |
micron | 4-5 or FS 6 micron |
Qc | slide with cryptcoccus |
Reagents in order | weigerts iron heme (soln' A&B) - nuclear stain, metanil yellow - counterstain, mucicarmine stain - stain mucin deep rose |
what are the A and B solutions of Weigerts comprised of | soln' A - hematox and alcohol, soln' B - HCL/ferric chloride/water |
what makes up the mucicarmine stain | carmin/alum lake, aluminum hydroxide (forms the chelation complex), ethyl alcohol (50 and 25%), anhydrous aluminum chloride |
results for Mucicarmine (cryptococcus) | mucin - deep rose to red, capsule of cryptococcus - deep rose to red, nuclei - black, other tissue - blue or yellow |
K.H. explaination of the principle | muci w/ aluminum mordant stains mucin to deep rose red. Aluminum is believed to for chelation agent binding acid groups of mucin and attaching red color to complex in a dye lake formation |
what can obscure carminophilic properties | overstaining with hematoxylin or metanil yellow |
what precaution must be used with anhydrous aluminum chloride | use under fume hood due to hydrogen chloride vapors |