Save
Busy. Please wait.
or

show password
Forgot Password?

Don't have an account?  Sign up 
or

Username is available taken
show password

why


Make sure to remember your password. If you forget it there is no way for StudyStack to send you a reset link. You would need to create a new account.
We do not share your email address with others. It is only used to allow you to reset your password. For details read our Privacy Policy and Terms of Service.


Already a StudyStack user? Log In

Reset Password
Enter the associated with your account, and we'll email you a link to reset your password.
focusNode
Didn't know it?
click below
 
Knew it?
click below
Don't know
Remaining cards (0)
Know
0:00
share
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

  Normal Size     Small Size show me how

UND 363 Mwave MSN

UND 363 Mwave Methenamine Silver Nitrate

QuestionAnswer
purpose for Mwave Methenamine Silver Nitrate rapid diagnosis of P jirovecii and pneumocystis corinii on cytospin preps or F/S's
Principle for Mwave Methenamine Silver Nitrate Fungal cell wall polysaccharides are oxidized to aldehydes by chromic acid. Cell walls/glycogen/mucins with large % polysacc.'s will remain reactive to methenimine-silver and reduced to visible.
fix cytospin preps fixes in 95% alcohol, FS fixed in 37-40% formaldehyde, standard paraffin sections with NBF
microns FS - 6 micron, paraffin - 4-5, **sections tend to wash off with this stain so charged slides should be used (poly l lysine or silanized slides)
QC use a control with the fungus you are looking for. chem. cleaned glassware and nonmetal forceps needed
reagents in order 10% chromic acid - oxidizer, soduim bisulfite 1% - remove chromic acid/clearing agent, methenimine silver - impregnation, gold chloride - toner, soduim thiosulfate - remove unreduced silver, lt green -counterstain
results fungi and P jirovecii cell wall - black, background - Green
how should the plastic coplin jars be used in the Mwave put the plastic jars in closed plastic bags, open microwaved bags under fume hood OR put mwave under fume hood
if slides are left too long in methenamine silver what will occur reticular fibers, RBCs and other tissue may be stained. The non specific staining may mimic fungi or obscure small fungi leading to potential false positives.
What is mirroring, what can cause it, FIX it? random deposits of silver onto slides. caused by over heating or dirty glassware. FIX - don't overheat, use ammoniacal silver for impregnation.
Created by: mustangvxd
 

 



Voices

Use these flashcards to help memorize information. Look at the large card and try to recall what is on the other side. Then click the card to flip it. If you knew the answer, click the green Know box. Otherwise, click the red Don't know box.

When you've placed seven or more cards in the Don't know box, click "retry" to try those cards again.

If you've accidentally put the card in the wrong box, just click on the card to take it out of the box.

You can also use your keyboard to move the cards as follows:

If you are logged in to your account, this website will remember which cards you know and don't know so that they are in the same box the next time you log in.

When you need a break, try one of the other activities listed below the flashcards like Matching, Snowman, or Hungry Bug. Although it may feel like you're playing a game, your brain is still making more connections with the information to help you out.

To see how well you know the information, try the Quiz or Test activity.

Pass complete!

"Know" box contains:
Time elapsed:
Retries:
restart all cards