Busy. Please wait.

show password
Forgot Password?

Don't have an account?  Sign up 

Username is available taken
show password


Make sure to remember your password. If you forget it there is no way for StudyStack to send you a reset link. You would need to create a new account.
We do not share your email address with others. It is only used to allow you to reset your password. For details read our Privacy Policy and Terms of Service.

Already a StudyStack user? Log In

Reset Password
Enter the associated with your account, and we'll email you a link to reset your password.
Don't know
remaining cards
To flip the current card, click it or press the Spacebar key.  To move the current card to one of the three colored boxes, click on the box.  You may also press the UP ARROW key to move the card to the "Know" box, the DOWN ARROW key to move the card to the "Don't know" box, or the RIGHT ARROW key to move the card to the Remaining box.  You may also click on the card displayed in any of the three boxes to bring that card back to the center.

Pass complete!

"Know" box contains:
Time elapsed:
restart all cards
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

  Normal Size     Small Size show me how

UND 363 Gridley

purpose for Gridley Fungus Stain demo fungi
principle chromic acid oxidizes adjacent glycol groups to aldehydes. Aldehydes then react to schiff reagent.
how is this principle different than the PAS stain chromic acid is a stronger oxidizer than periodic acid and will destroy/turn some aldehydes to acid therefore leaving less reactive groups to react with schiff. (less intense reaction than PAS but also less background staining.
In addition to the chromic acid destroying many aldehyde groups what will the aldehyde fuchsin do in its place. the aldehyde fuchsin will act as an aldehyde and occupy uninvolved linkages of the schiff reaget thereby reinforcing the depth of the stain.
fix NBF
micron 4-5
QC section with fungus
reagents in order 4% chromic acid - oxidizer, Schiff - reacts with aldehydes, aldehyde fuchsin - acts as aldehyde by occupying uninvolved linkages of schiff reagent, metanil yellow - counterstain
results mycelia/elastic fibers/mucin - deep purple, conidia - deep rose to purple, background - yellow
how will old non-viable fungi be stained with this technique? they are not well stained if they are non-viable
what type of acid can be used after the schiff reagent sulfurous acid - will remove excess schiff reagent
of pararosaniline (CI42500) and basic fuchsin (rosaniline CI 42510) which is use to prepar the aldehyde fuchsin pararosaniline
explain in detail what the chromic acid will do the glycols (in trace amts. Vs. those with heavy polysaccharide %) oxidizes 1,2 glycols to aldehydes and then oxidizes some further to acid (trace amts.) Tissue with heavy polysaccharides (mucin, glycoten, fungi) take longer and will still show + PAS RXN even after basement membrane and conn. tissue are non-reactive.
Created by: mustangvxd