click below
click below
Normal Size Small Size show me how
UND 363 Mwave Z/Neel
UND 363 Mwave Ziehl Neelsen
Question | Answer |
---|---|
purpose For Mwave Ziehl Neelsen (same as kinyoun except for a few differences) | demo of acid fast mycobacteria |
principle | lipoid capsule of A/F mycobacteria takes up carbol fuchsin and resists decolorization with dilute mineral ACID. |
what is carbol fuchsin more soluble in (lipids of cell wall or acid-alcohol) and how does this affect NON A/F bacteria | it is more soluble in the lipids of the cell wall. NON A/F bacteria lack the waxy capsule and therefore carbol-fuchsin is easily removed |
what does the Phenol and alchol in this stain do? | they aid in dissolving the basic fuchsin |
fix | NBF preferred, others can be used EXCEPT CARNOYS |
what does Carnoys fixative do to A/F bacteria | It will make them NON A/F |
microns | 4-5 |
why can tap or distilled water NOT BE USED before the carbol-fuchsin step | there may be bacteria in the piping |
Qc | tissue with A/F organisms, must use Millipore filtered (.45 micron pore size) water, and a negative control from same day workload |
what does the carbol fuchsin do? what about the Phenol/alcohol? | C.F. stains the lipid capsule, the phenol and alcohol enhances staining and aids in dissolving of the stain. |
what must be done after the adic alcohol step (hcl/alcohol) | must be rinsed well before counterstaining or the tissue will not stain |
why can the sections not be allowed to dry out after carbol fuchsin | a compound that resists decolorization will form |
what helps the penetration of phenol (AKA carbolic acid) in this stain | heat and the action of wetting agents (which reduce surface tension and enhance spread of phenol) |
if overstained with methylene blue what will occur and what can be done | it will mask any organisms present. Fix - take slide back to acid alcohol (step 4) to remove methylene blue, wash with water and repeat counterstain |
what are the 3 differences between Ziehl Neelsen Mwave Tech and Kinyoun | 1) Different Quantities .8g fuchsin, 7.5ml phenol, 14ml isopropyl VS 28g/56ml/140ml 95%alcohol, 2) uses ISOPROPYL ALCOHOL instead of 95% or absolute, 3)Time is less |
what is the difference in acid alcohol in this stain Vs. Kinyoun | .5% Vs the 1.0% in kinyoun |
what is added to the methylene blue solution that is different from kinyoun and the regular ziehl-neelsen stains | acetic acid |
results | mycobacteria - red, RBC's - pink - Mast Cells - blue, other tissue elements - Pale Blue |
what can carbol fuchsin be prepared with that will enhance its staining of A/F bacteria | pararosaniline, rosaniline, magenta II, new fuchsin, or mixtures of these dyes |
why must the slides be diped up and down in this solution | to keep the temperature equal throughout the solution |
what is the temperature difference for this stain Vs kinyoun and the regular Ziehl Neelsen stain | can be done at room temperature by staining sections for 30 min in the carbol-fuchsin |