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UND 363 Mwave Warthi
UND 363 Mwave Warthin Starry
Question | Answer |
---|---|
purpose for Mwave Warthin Starry | demo spirochetes and other bacteria |
principle for Mwave Warthin Starry | argyrophil method (ability to bind or be impregnated with silver ions but require reducing agent) - spirochetes can bind silver but need hydroquinone to reduce to visible |
what occurs to the hydroquinone via reduction | it is oxidized to quinone |
fixative | NBF |
microns | 4-5 |
QC for Mwave Warthin Starry | tissue must contain backeria, MUST USE chemically clean glasswar, nonmetalic forceps |
reagents in order for Mwave Warthin Starry | glycine acetic acid soln. PH is approx 3.9 silver nitrate - impregnator silver nitrate/gelatin/hydroquinone mix - developer |
results for Mwave Warthin Starry | alipis felis (cat scratch bacillus)/legionella/nocardia/H. pylori - black, Nuclei/erythrocytes - Brown |
why is gelatin type so important for Mwave Warthin Starry | using BBL bacterial gelatin or fisher typ a gave less background staining |
what will occur if xylene substitutes are used | will cause rapid fading of the stain |
when using the Mwave method what helps create the most consistent results | using low power levels of heat |
If using this stain for spirochetes what modifications must be done for Mwave Warthin Starry | after step 1 (glycine acetic acid ph 3.9), sections are put in uranyl nitrate and acetic acid/glycine mix and micro (lvl 7) for 1 min. + 1 min. standing, rinse with 5 changes of working acetic acid glycine mix, go to step 3 (ie impregnation/developer) |