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UND 363 Mwave Warthi

UND 363 Mwave Warthin Starry

QuestionAnswer
purpose for Mwave Warthin Starry demo spirochetes and other bacteria
principle for Mwave Warthin Starry argyrophil method (ability to bind or be impregnated with silver ions but require reducing agent) - spirochetes can bind silver but need hydroquinone to reduce to visible
what occurs to the hydroquinone via reduction it is oxidized to quinone
fixative NBF
microns 4-5
QC for Mwave Warthin Starry tissue must contain backeria, MUST USE chemically clean glasswar, nonmetalic forceps
reagents in order for Mwave Warthin Starry glycine acetic acid soln. PH is approx 3.9 silver nitrate - impregnator silver nitrate/gelatin/hydroquinone mix - developer
results for Mwave Warthin Starry alipis felis (cat scratch bacillus)/legionella/nocardia/H. pylori - black, Nuclei/erythrocytes - Brown
why is gelatin type so important for Mwave Warthin Starry using BBL bacterial gelatin or fisher typ a gave less background staining
what will occur if xylene substitutes are used will cause rapid fading of the stain
when using the Mwave method what helps create the most consistent results using low power levels of heat
If using this stain for spirochetes what modifications must be done for Mwave Warthin Starry after step 1 (glycine acetic acid ph 3.9), sections are put in uranyl nitrate and acetic acid/glycine mix and micro (lvl 7) for 1 min. + 1 min. standing, rinse with 5 changes of working acetic acid glycine mix, go to step 3 (ie impregnation/developer)
Created by: mustangvxd