Busy. Please wait.

show password
Forgot Password?

Don't have an account?  Sign up 

Username is available taken
show password


Make sure to remember your password. If you forget it there is no way for StudyStack to send you a reset link. You would need to create a new account.
We do not share your email address with others. It is only used to allow you to reset your password. For details read our Privacy Policy and Terms of Service.

Already a StudyStack user? Log In

Reset Password
Enter the associated with your account, and we'll email you a link to reset your password.
Don't know
remaining cards
To flip the current card, click it or press the Spacebar key.  To move the current card to one of the three colored boxes, click on the box.  You may also press the UP ARROW key to move the card to the "Know" box, the DOWN ARROW key to move the card to the "Don't know" box, or the RIGHT ARROW key to move the card to the Remaining box.  You may also click on the card displayed in any of the three boxes to bring that card back to the center.

Pass complete!

"Know" box contains:
Time elapsed:
restart all cards
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

  Normal Size     Small Size show me how

UND 363 Mwave Warthi

UND 363 Mwave Warthin Starry

purpose for Mwave Warthin Starry demo spirochetes and other bacteria
principle for Mwave Warthin Starry argyrophil method (ability to bind or be impregnated with silver ions but require reducing agent) - spirochetes can bind silver but need hydroquinone to reduce to visible
what occurs to the hydroquinone via reduction it is oxidized to quinone
fixative NBF
microns 4-5
QC for Mwave Warthin Starry tissue must contain backeria, MUST USE chemically clean glasswar, nonmetalic forceps
reagents in order for Mwave Warthin Starry glycine acetic acid soln. PH is approx 3.9 silver nitrate - impregnator silver nitrate/gelatin/hydroquinone mix - developer
results for Mwave Warthin Starry alipis felis (cat scratch bacillus)/legionella/nocardia/H. pylori - black, Nuclei/erythrocytes - Brown
why is gelatin type so important for Mwave Warthin Starry using BBL bacterial gelatin or fisher typ a gave less background staining
what will occur if xylene substitutes are used will cause rapid fading of the stain
when using the Mwave method what helps create the most consistent results using low power levels of heat
If using this stain for spirochetes what modifications must be done for Mwave Warthin Starry after step 1 (glycine acetic acid ph 3.9), sections are put in uranyl nitrate and acetic acid/glycine mix and micro (lvl 7) for 1 min. + 1 min. standing, rinse with 5 changes of working acetic acid glycine mix, go to step 3 (ie impregnation/developer)
Created by: mustangvxd