Busy. Please wait.

show password
Forgot Password?

Don't have an account?  Sign up 

Username is available taken
show password


Make sure to remember your password. If you forget it there is no way for StudyStack to send you a reset link. You would need to create a new account.
We do not share your email address with others. It is only used to allow you to reset your password. For details read our Privacy Policy and Terms of Service.

Already a StudyStack user? Log In

Reset Password
Enter the associated with your account, and we'll email you a link to reset your password.
Don't know
remaining cards
To flip the current card, click it or press the Spacebar key.  To move the current card to one of the three colored boxes, click on the box.  You may also press the UP ARROW key to move the card to the "Know" box, the DOWN ARROW key to move the card to the "Don't know" box, or the RIGHT ARROW key to move the card to the Remaining box.  You may also click on the card displayed in any of the three boxes to bring that card back to the center.

Pass complete!

"Know" box contains:
Time elapsed:
restart all cards
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

  Normal Size     Small Size show me how

UND 363 Warthin Star

UND 363 Warthin Starry

purpose for Warthin Starry demo spirochetes
Principle for Warthin Starry argyrophil method (ability to bind or be impregnated with silver ions but require reducing agent) - spirochetes can bind silver but need hydroquinone to reduce to visible
fix for Warthin Starry NBF
microns 4-5
QC for Warthin Starry tissue with spirochetes (MUST use new plastic centrifuge tubes and chemically clean glassware and non metalic forceps
4 reagents in order for Warthin Starry 1)adiculated water (h20 and citric acid) - must be ph 4 for spirochetes, 2)silver nitrate (1%) - impregnation, 3)hydroquinone - reducer, 4)developer - silver nitrate, gelatin, hydroquinone (reducer)
resultes spirochetes/other bacteria - black, background pale yellow to lt brown
If underdeveloped what will the stain appear as and the FIX yellow instead of black, FIX - develop new slides, or rehydrate and develop with fresh mixture
If OVERdeveloped what occurs and what can be done FIX -treat with iodine and sodium thiosulfate to remove color and then restain therefore it will non-selectively blacken ALL bacteria (use as screening method)
what occurs to the hydroquinone via reduction it is oxidized to quinone
Created by: mustangvxd