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UND 363 Diff Quick
UND 363 Diff Quick Giemsa
| Question | Answer |
|---|---|
| purpose For Diff Quik Giemsa | stain heliobacter pylori (H. Pylori) A flagellated, spiral shaped baceria linked to ulcers and carcinoma |
| principle For Diff Quik Giemsa | COMMERCIALLY MADE modified romanowsky stain - "neutral dyes combine with basic dye methylene blue and acid dye Eosin" |
| if the ph is more alkaline what will occur to the methylene blue | it will give rise to new substances that are metachromatic |
| what is typically used instead of methyl violet in this commercial stain | weighed amounts of Azures (it has both azure A and methylene blue in its "solution 2" |
| fixative For Diff Quik Giemsa | formalin |
| micron | 4-5 |
| QC For Diff Quik Giemsa | sections with heliobacter Pylori (H. Pylori) |
| reagents in order | "solution 1" (eosin y buffered) stains cyto red, "Solution 2" (Azure A and methylene blue mix) - stains nuclei and bacteria blue, Acetic adic water - Diff'r |
| what is the purpose of the Fixative solution in the Commercial Diff Quick Giemsa | it is not used in histology |
| results For Diff Quik Giemsa | H. pylori/nuclei - dk. blue, other bacteria - blue, cytoplasm - pink |
| what will occur if the stained slides are left in the last distilled water or in dehydrating alcohol too long | it will lead to excess decolorization. |
Created by:
mustangvxd
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