Busy. Please wait.
Log in using Clever

show password
Forgot Password?

Don't have an account?  Sign up 
Sign up using Clever

Username is available taken
show password


Make sure to remember your password. If you forget it there is no way for StudyStack to send you a reset link. You would need to create a new account.
Your email address is only used to allow you to reset your password. See our Privacy Policy and Terms of Service.

Already a StudyStack user? Log In

Reset Password
Enter the associated with your account, and we'll email you a link to reset your password.
Didn't know it?
click below
Knew it?
click below
Don't know
Remaining cards (0)
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

  Normal Size     Small Size show me how

UND 363 Giemsa

UND 363 May Grunwald Giemsa

purpose for Giemsa stain differentiation of cells in hematopoietic tissue (ie where cells and other elements are formed) AND to demo microorganisms
principle for Giemsa stain Is Modified Romanovsky Stain - neutral dyes combin with basic dye AZURE B and adic dye EOSIN Y to give wide color range (polychrome stain)
What stains are combined for Giemsa stain Azure B (basic) and Eosin Y (acidic)
how does PH affect the giemsa stain it will cause variation of the staining characteristics (IE more acid will give more chromatin staining and less cytoplasmic; less acid will give denser nuclei and increase cyto basophilia)
fixative for Giemsa stain Zenkers or B5 preferred, but NBF is ok
What can Zenkers do in this stain fixes and Decalcifies bone marrow
microns 3-4
control for Giemsa stain spleen section
6 reagents in order for Giemsa stain if 1)zenkers/B5 used then 2)lugol iodine/sodium thiosulfate to remove mercury pigmen 3)methanol - fix, 4) jenner, 5)giemsa, 6)acetic acid then 95% alcohol- diffr'
why is a secondary diff'r used seperately (95% alcohol) besides the acetic acid it is due to the Eosin Y in the giemsa stain
results for Giemsa stain nuclei - blue, WBC/cytoplasm - pink/gray/or blue, bacteria - BLUE
why is the cytoplasm color varied in the results it is due to cell type and development
Why should the reagents be made just before use the working solutions are not stable
why is ph adjustment needed in this stain it needs to be adjusted for different fixatives. (IE more acid will give more chromatin staining and less cytoplasmic; less acid will give denser nuclei and increase cyto basophilia)
what is the typical ph for giemsa stain 6.4 - 6.9
if there is an increaced acidity how will the results vary it will decrease basophilia and the RBC's will be pink and the WBC's will be understained
if there is a decrease in acid how will the results vary there will be a increased basophilia and the RBC's will be blue gray, WBC nuclei will appear more densly stained.
Created by: mustangvxd



Use these flashcards to help memorize information. Look at the large card and try to recall what is on the other side. Then click the card to flip it. If you knew the answer, click the green Know box. Otherwise, click the red Don't know box.

When you've placed seven or more cards in the Don't know box, click "retry" to try those cards again.

If you've accidentally put the card in the wrong box, just click on the card to take it out of the box.

You can also use your keyboard to move the cards as follows:

If you are logged in to your account, this website will remember which cards you know and don't know so that they are in the same box the next time you log in.

When you need a break, try one of the other activities listed below the flashcards like Matching, Snowman, or Hungry Bug. Although it may feel like you're playing a game, your brain is still making more connections with the information to help you out.

To see how well you know the information, try the Quiz or Test activity.

Pass complete!

"Know" box contains:
Time elapsed:
restart all cards