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UND 363 Lux Fast Blu
UND 363 Luxol Fast Blue
| Question | Answer |
|---|---|
| purpose | demo myelin |
| principle | stains due to lipoproteins. it is an adic-base reaction that forms salt. the base of lipoprotein replaces the base of the dye. |
| what is unique about luxol fast blue | is of the sulfonated copper phthalocyanine dye type but is alchohol soluble |
| fix | formalin |
| tech | 10-15 |
| qc | spinal cord or medulla |
| procedure/reagents in order | luxol fast blue (alcohol/sulfonated copper stain), lithium carbonate (diff'r), 70% alcohol (diff'r), |
| results | myelin - blue to blue/green, background - colorless |
| grey matter and demylinated white matter should be what color compared to myelinated white matter | grey matter and demyelinated white should be colorless and myelinated white should be blue |
| if the grey matter id darker than the white matter what should be done | continue differentiation until the grey matter is colorless and white matter is blue |
| typically, if the stain is done correctly, what is the best way to id/gross id the stain | macroscopically |
Created by:
mustangvxd
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