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UND 363 Weil
| Question | Answer |
|---|---|
| purpose | demo myelin |
| principle | regressive stain with a mordant/hematoxylin sol'n. It is diff'd in 2 steps. 1st diff is done macroscopically with ferric ammoniom sulfate (excess mordant diff'r), the second is done microscopically with borax ferricyanide (oxidizer diff'r) |
| how is the first diff'r done | uses ferric ammoniom sulfate to remove excess dye via "excess mordant differentiation" macroscopically |
| how is the second diff'r done | microscopically via borax ferricyanide (oxidizer diff'r) which removed any remaining no specifically bound hematox lake and forms colorless oxidation product |
| fix | formalin |
| tech | 10-15 |
| qc | spinal cord or medulla (w/ myelinate areas) |
| reagents in procedure | ferric ammoniom sulfate (mordant)\alcoholic hematox (stain), ferric ammoniom sulfate (diff'r macro), soduim borate/potassium ferricyanide sol'n (diff'r micro) |
| results | myelin sheath - blue to blue-black, background - lt tan |
| how should grey matter/demyelinated white matter differ in color from the myelinated white matter | grey matter/demyelinated white should be lt brown and contrast sharply with the blue-black appearance of myelinated white matter |
| if the myelin stain is done well how can the demyelinated be identified | more easily macroscopically than Micro. |
| what can be done with excess hematox if it is not done very often | the alocoholic hematox can be measured and diluted to be used in other staining protocols such as weigert hematoxylin |
| what is the mordant in this stain | ferric alum |
Created by:
mustangvxd
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