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UND 363 Holzer

purpose demo glial fibers and gliosis
define gliosis excessive proliferation of glial cells and/or processes
principle glial fibers stained with crystal violet and then become resistant to decolorization with the alkaline aniline-chloroform differentiating mixture.
fix formalin
tech 6-8
qc cerebral cortex (not spinal cord)
what does the differentiation soln' consist of aniline oil, chloroform, ammonium hydroxide
reagent and uses in order of procedure phophomolybdic acid (mordant), alcohol chloroform mix (makes tissue translucent), crystal violet (stains tissue blue), potassium bromide (rinse), differentiate (aniline oil, chloroform, ammonium hydroxide mix)`
results glial fibers - blue, background - very pale blue to colorless
what can remove the a crystal violet precipitate straight aniline oil
what care must be taken with aniline oil is a sensitizer, toxic via skin, fume hood advisible, ppm of 5
what is used instead of the holzer method now IHC
Created by: mustangvxd