Busy. Please wait.
or

show password
Forgot Password?

Don't have an account?  Sign up 
or

Username is available taken
show password

why


Make sure to remember your password. If you forget it there is no way for StudyStack to send you a reset link. You would need to create a new account.
We do not share your email address with others. It is only used to allow you to reset your password. For details read our Privacy Policy and Terms of Service.


Already a StudyStack user? Log In

Reset Password
Enter the associated with your account, and we'll email you a link to reset your password.
Don't know
Know
remaining cards
Save
0:01
To flip the current card, click it or press the Spacebar key.  To move the current card to one of the three colored boxes, click on the box.  You may also press the UP ARROW key to move the card to the "Know" box, the DOWN ARROW key to move the card to the "Don't know" box, or the RIGHT ARROW key to move the card to the Remaining box.  You may also click on the card displayed in any of the three boxes to bring that card back to the center.

Pass complete!

"Know" box contains:
Time elapsed:
Retries:
restart all cards
share
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

  Normal Size     Small Size show me how

UND 363 Holzer

QuestionAnswer
purpose demo glial fibers and gliosis
define gliosis excessive proliferation of glial cells and/or processes
principle glial fibers stained with crystal violet and then become resistant to decolorization with the alkaline aniline-chloroform differentiating mixture.
fix formalin
tech 6-8
qc cerebral cortex (not spinal cord)
what does the differentiation soln' consist of aniline oil, chloroform, ammonium hydroxide
reagent and uses in order of procedure phophomolybdic acid (mordant), alcohol chloroform mix (makes tissue translucent), crystal violet (stains tissue blue), potassium bromide (rinse), differentiate (aniline oil, chloroform, ammonium hydroxide mix)`
results glial fibers - blue, background - very pale blue to colorless
what can remove the a crystal violet precipitate straight aniline oil
what care must be taken with aniline oil is a sensitizer, toxic via skin, fume hood advisible, ppm of 5
what is used instead of the holzer method now IHC
Created by: mustangvxd