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UND 363 Holzer
Question | Answer |
---|---|
purpose | demo glial fibers and gliosis |
define gliosis | excessive proliferation of glial cells and/or processes |
principle | glial fibers stained with crystal violet and then become resistant to decolorization with the alkaline aniline-chloroform differentiating mixture. |
fix | formalin |
tech | 6-8 |
qc | cerebral cortex (not spinal cord) |
what does the differentiation soln' consist of | aniline oil, chloroform, ammonium hydroxide |
reagent and uses in order of procedure | phophomolybdic acid (mordant), alcohol chloroform mix (makes tissue translucent), crystal violet (stains tissue blue), potassium bromide (rinse), differentiate (aniline oil, chloroform, ammonium hydroxide mix)` |
results | glial fibers - blue, background - very pale blue to colorless |
what can remove the a crystal violet precipitate | straight aniline oil |
what care must be taken with aniline oil | is a sensitizer, toxic via skin, fume hood advisible, ppm of 5 |
what is used instead of the holzer method now | IHC |