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UND 363 Mallory PTAH

purpose demo of glial fibers
principle phosphotungstic acid is 20:1 over hematein. tungsten binds hemetein to blue lake. Lake gives blue color to (glial fiber, nuclei, myelin), phosphotungstic acid stains neurons red-brown/salmon
what is a lake mordant + dye
what type of stain is this ____________chrome polychrome
fix formalin
tech 6-8
qc cerebral cortex, NOT spinal cord
why would components in this stain lose their red-brown color they were washed in water or prolonged alcohol washings, therefore the dehydration needs to be rapid.
what are the reagents in the procedure (step by step) mordant with zenkers (containing acetic acid),lugol iodine to remove mercury pigment, alcohol to decolorize, pot. permanganate to oxidize, oxalic acid to decolorize, PTAH (polychrome stain)
results glial fibers/nuclei/myelin - blue, neurons - salmon
notes holzer method is a better stain, but both have been replaced by IHC methods
what does the mordant w/ zenkers do enhances staining of glia
how does this stain differ from regular PTAH the slides are not taken through sodium thiosulfate.
Created by: mustangvxd