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UND 363 SevierMunger
UND 363 Sevier Munger Biel Mod
Question | Answer |
---|---|
purpose | demo nerve fibers, nerofibrillary tangles, senile plaques in alzheimer pt's |
principle | silver impregnation on neurofibrils and axons, reduced via formaldehyde. soduim thiosulfate excess silver removal |
does this stain use gold for a toner, and what is the result | no it does not therefore the background stains yellow |
does this silver stain need chemically cleaned glassware? | NO. |
tech | 6-8 |
qc | CNS tissue |
results | nerve endings/neurofibrils/neurofibrillary tangles and peripheral neurites of neuritic plaque - black, background - yellow |
why is the proportion of ammonium hydroxide and formalin critical | the concentration and relative proportions are critical to controlled development of the stain |
what must be left after the first addition of ammonium hydroxide | some grains of silver must be left in the flask after ammonium hydroxide. excess ammonia must NOT be added. ie the ammoniacal silver solution prep must be slightly cloudy at its end point. |
what is this stain also useful for besides nerves | it is an argyrophil stain good for demonstrating granules of some carcinoid tumor cells. |