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UND 363 Biel M'wave

UND 363 Bielschowsky Mwave

purpose for biel m'wave demo nerve fibers and neurofirillary tangles/senile plaques
principle impregnation with ammoniacal silver, recudtion from formaldehyde, NOT Toned w/ GOLD, sodium thiosulfate for excess silver removal
what will occur since gold chloride is not used in this method the background will remain yellow.
fix formalin
tech 8 micron
qc CNS must be used (tissue should contain senile plaques and neurofibrillary tangles from alzheimer pt. if possible)
results axon/cytoplasmic neurofibrils - brown to black, neurofibrillary tangles and plaques (from alzheimer pt's) - dk brown or black, neromelanin - black, lipofuscin - brown or black, blackground - yellow
notes must use chem cleaned glassware that is rinsed twice in distilled water.
what is positive about this stain method requires much less silver nitrate
Created by: mustangvxd



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