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UND 363 Biel PAS

UND 363 Bielschowsky PAS

Purpose for neurofibrillary tangles and senile plaques in alzheimer disease
principle ammoniacal silver impregnation (neurofibrils/axons), reduction via formaldehyde, gold toned, soduim thio to remove excess silver, schiff to stain basement membrane and amyloid in plaque
what will/will not occur because gold choloride was used the background will not become yellow
explain the PAS reaction Periodic acid oxidizes elements (glycols) to aldehydes, the schiff reagent reacts with aldehydes to produce a magenta color
fix formalin
equip chem. cleaned
tech 8-10 micron
qc tissue from CNS, should contain senile plaques and neurofibrillary tangles
procedure ammoniacal silver impregnation, formaldehyde developed, gold toned, soduim thio silver removal, periodic acid oxidation, schiff reaction to aldehydes to form color
results neurofibrillary tangles/peripheral neurites of neuritic plaques/axons - dk black, amyloid/lipofuscin - magenta
what is another name for neuritic plaques senile plaques or amyloid plaques
Created by: mustangvxd



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