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UND 363 Holmes Silve

UND 363 Holmes Silver

purpose for holmes Silver nitrate demo nerve fibers and neurofibrils
holmes silver is a mod of the bodian tech. what is the difference? bodian was inconsistent, holmes found that it wasn't alkaline enough for optimal impregnation
fix formalin
equip chem cleaned glass
tech 10-15 micron
qc cerbral cortex (spinal cord not good because axons appear in cross section)
principle (changes from bodian tech.) alkaline impregnation soln' (boric acid, soduim borate, silver nitrate, pyridine) and a reducing solution (hydroquinone and sodium sulfate)
what makes the impregnation solution alkaline and what is its purpose pyridine, changes the electrostatic condition of the tissue.
procedure impregnate with alkaline soln, reducer (hydroquinone/soduim sulfite), tone with gold chloride, oxalic acid (reduces gold chloride and gives purple tinge), soduim sulfate to remove excess silver)
results axons, nerve fibers, neurofibrils - black
Created by: mustangvxd



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