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UND 363 Holmes Silve
UND 363 Holmes Silver
Question | Answer |
---|---|
purpose for holmes Silver nitrate | demo nerve fibers and neurofibrils |
holmes silver is a mod of the bodian tech. what is the difference? | bodian was inconsistent, holmes found that it wasn't alkaline enough for optimal impregnation |
fix | formalin |
equip | chem cleaned glass |
tech | 10-15 micron |
qc | cerbral cortex (spinal cord not good because axons appear in cross section) |
principle (changes from bodian tech.) | alkaline impregnation soln' (boric acid, soduim borate, silver nitrate, pyridine) and a reducing solution (hydroquinone and sodium sulfate) |
what makes the impregnation solution alkaline and what is its purpose | pyridine, changes the electrostatic condition of the tissue. |
procedure | impregnate with alkaline soln, reducer (hydroquinone/soduim sulfite), tone with gold chloride, oxalic acid (reduces gold chloride and gives purple tinge), soduim sulfate to remove excess silver) |
results | axons, nerve fibers, neurofibrils - black |