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UND 363 Bodian
| Question | Answer |
|---|---|
| purpose for bodian | stain nerve fibers |
| principle for bodian | "protargol" (silver) impregnates tissue, copper (added to progargol) destains conn. tissue, hydroquinone/formalin reduce, gold toned, oxalic acid to reduce gold/intensify stain, sodium thio. remove unreduced silver, aniline blue counterstains |
| what further help does the copper added to the protargol do? | destains connective tissue which will further diff'r between neural and connective tissues |
| why was formalin added to the hydroquinone silver reducer | luna found it to give more consistent results than soduim sulfite. |
| fix | formalin |
| why must chem. cleaned glassware be used | a black precipitate will form during stains with impegnation techniques |
| qc | peripheral nerve or cerbral cortex. spinal cord is NOT GOOD because most nerve fibers will appear in cross section. |
| besides chem clean glassware what type of forceps should be used | non metallic |
| tech | 6-8 microns |
| results | nerve - black, background - lt gray or blue, nuclei - black |
| what is aqua regia used for | cleans the copper shot that is added to the progargol impregnation solution |
| how is aqua regia disposed of | poured into LARGE volume of water and then discarded in the sink (under fume hood) |
| notes | protargol must be left undisturbed until dissolved, do not over counterstain with aniline blue or contrast will be lost |
| if it is difficult to differentiate between blue and black (microscopally) what can be used instead | nuclear fast red |
Created by:
mustangvxd
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