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UND 363 Cresl vacca

UND 363 Cresyl Echt vacca

purpose of Cresyl Echt Vacca demo nissl substance (chromatolysis ie injury and subsequent loss)
principle Cresyl Echt Vacca Nissl susbstance is basophilic and stains sharply with basic aniline dyes. Varying the PH and differentiation, nissl substance and nuclei will stain or only nissl substance will demo.
what is the difference in principle from the Vacca method from the Luna method Vacca uses cresyl violet at an acid PH (due to acetic acid in the working solution)
fix formalin
tech 6-8
qc spinal cord
reagents in order cresyl echt violet/acetic acid mix (aniline dye)
results nissl/nuclei - blue-purple, background - colorless
notes appear unstained macroscopically (can lead to false conclusions, check with microscope)
what 3 types of cresyl can be used interchangeably with this tech cresyl fast violet, cresyl echt violet, cresyl violet acetate
Created by: mustangvxd



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