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UND 363 Cresl E Luna

UND 363 Cresyl Echt luna

purpose Cresyl Echt Luna demo nissl substance (chromatolysis ie injury and subsequent loss)
Principle Cresyl Echt Luna Nissl susbstance is basophilic and stains sharply with basic aniline dyes. Varying the PH and differentiation, nissl substance and nuclei will stain or only nissl substance will demo.
fix 10% NBF
tech 6-8 micron
QC spinal cord
reagents in order cresyl echt (aniline dye), balsam-xylene (diffr'), absolute (diffr')
results nissl/nuclei (blue to purple), background (colorless
notes should be repeated until background is colorless, alcohol following balsam-xylene will become cloudy and needs frequent changing
Created by: mustangvxd