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UND 363 Cresl E Luna
UND 363 Cresyl Echt luna
Question | Answer |
---|---|
purpose Cresyl Echt Luna | demo nissl substance (chromatolysis ie injury and subsequent loss) |
Principle Cresyl Echt Luna | Nissl susbstance is basophilic and stains sharply with basic aniline dyes. Varying the PH and differentiation, nissl substance and nuclei will stain or only nissl substance will demo. |
fix | 10% NBF |
tech | 6-8 micron |
QC | spinal cord |
reagents in order | cresyl echt (aniline dye), balsam-xylene (diffr'), absolute (diffr') |
results | nissl/nuclei (blue to purple), background (colorless |
notes | should be repeated until background is colorless, alcohol following balsam-xylene will become cloudy and needs frequent changing |