Busy. Please wait.

show password
Forgot Password?

Don't have an account?  Sign up 

Username is available taken
show password


Make sure to remember your password. If you forget it there is no way for StudyStack to send you a reset link. You would need to create a new account.
We do not share your email address with others. It is only used to allow you to reset your password. For details read our Privacy Policy and Terms of Service.

Already a StudyStack user? Log In

Reset Password
Enter the associated with your account, and we'll email you a link to reset your password.
Didn't know it?
click below
Knew it?
click below
Don't know
Remaining cards (0)
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

  Normal Size     Small Size show me how

UND 363 Toluid Blue

UND 363 Toluidine Blue

Purpose for Toluidine Blue demo of mast cells (connective tissue cells)
principle mast cells stain metachromatic with toluidine (stain diff than stain color)
what will the control section stain colorwise mast cells red-purple, background - blue (orthochromatic)
what is metachromasia dependant on attributed to cationic (basic dye), is partly depandant on PH, dye conc. and temp.
fix for Toluidine Blue 10% NBF
tech 4-5 microns
QC need a section that has mast cells for control.
reagents used toluidine blue - metachromatic stain, alcohol - dehydrant
why can alcohol be used in this stain when most metachromatic stains it cannot mast cell granules are stable and will not be lost after alcoholic dehydration
how can a rapid screening be done for mast cells apply a light stain of methylene blue (mast cells will be well demo'd)
what other stain can be used for mast cells methyl green-pyronin
Created by: mustangvxd



Use these flashcards to help memorize information. Look at the large card and try to recall what is on the other side. Then click the card to flip it. If you knew the answer, click the green Know box. Otherwise, click the red Don't know box.

When you've placed seven or more cards in the Don't know box, click "retry" to try those cards again.

If you've accidentally put the card in the wrong box, just click on the card to take it out of the box.

You can also use your keyboard to move the cards as follows:

If you are logged in to your account, this website will remember which cards you know and don't know so that they are in the same box the next time you log in.

When you need a break, try one of the other activities listed below the flashcards like Matching, Snowman, or Hungry Bug. Although it may feel like you're playing a game, your brain is still making more connections with the information to help you out.

To see how well you know the information, try the Quiz or Test activity.

Pass complete!

"Know" box contains:
Time elapsed:
restart all cards