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UND 363 Toluid Blue

UND 363 Toluidine Blue

QuestionAnswer
Purpose for Toluidine Blue demo of mast cells (connective tissue cells)
principle mast cells stain metachromatic with toluidine (stain diff than stain color)
what will the control section stain colorwise mast cells red-purple, background - blue (orthochromatic)
what is metachromasia dependant on attributed to cationic (basic dye), is partly depandant on PH, dye conc. and temp.
fix for Toluidine Blue 10% NBF
tech 4-5 microns
QC need a section that has mast cells for control.
reagents used toluidine blue - metachromatic stain, alcohol - dehydrant
why can alcohol be used in this stain when most metachromatic stains it cannot mast cell granules are stable and will not be lost after alcoholic dehydration
how can a rapid screening be done for mast cells apply a light stain of methylene blue (mast cells will be well demo'd)
what other stain can be used for mast cells methyl green-pyronin
Created by: mustangvxd
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