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UND 363 Sudan Black

UND 363 Sudan Black B

purpose for Sudan Black B demo neutral lipids
why is sudan black especially useful most sensitive of lipid dyes. more soluble in phospholipids and cerebrosides Vs. Oil red O.
how can sudan black be used for leukocytes aids in diff'ing granulocyte precursors from leukocytes committed to lymphocytic or monocytic pathways
principle for Sudan Black B soluble in neutral fats. Is slightly basic dye and will combine with acidic groups in compound lipids (therefore will stain phospholipids)
fix for Sudan Black B 10%NBF, or sections post fixed in calcium formalin (cannot use alcoholic fixatives)
tech 10 micron FS's
QC not needed most tissue contains fat
results for Sudan Black B fat - blue-black, nuclei - red
reagents (in order) 100% proplylene glycol - dehydration (if not previously fixed then calcuim formol to prop glycol), sudan black b - fat stain, 85% propylene glycol - diff's, nuclear fast red - counter stain, aqueous mount
note are same as oil red o free float sections best, adding 30% sucrose before freezing, glycerin jelly temp, coverslipping issues
Created by: mustangvxd