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UND 363 P.A. Meth.

UND 363 Periodic Acid-Methenamine silver

QuestionAnswer
Purpose for Periodic Acid-Methanamine silver M'wave best delineates basement membrane (used in detection of abnormalities of glomerular BM in KIDNEY)
principle for Periodic Acid-Methanamine silver M'wave periodic acid oxidize carbs of basement membrane which reduces aldehyde. The least aldehydes will be reduced w/ silver to visible metallic silver
books principle for Periodic Acid-Methanamine silver M'wave carbs of BM oxidized to aldehyde by Per. Acid. Aldehydes bind to silver ions from methen. silver and reduce silver to visible. Toned with gold chloride and unreduced silver removed with sodium thiosulfate
fix for Periodic Acid-Methanamine silver M'wave 10% NBF preferred, CANNOT use mercury containing fixatives.
tech ***2 microns****
QC for Periodic Acid-Methanamine silver M'wave kidney as internal control, no other control is necessary
reagents for Periodic Acid-Methanamine silver M'wave (in order) periodic acid - oxidation, methanimine silver - silver impregnation, gold chloride - toning, sodium thiosulfate - remove unreduced silver, lt green - counterstain
results basement membrane - black, background - green
why is m'wave used give sharper stain of BM and less background staining
why is temp critical should be just below or approx. 95○C (each oven should be calibrated for appropriate time)
what are some issues with this stain very difficult to perform correctly, stopping silver staining too soon will result in uneven or interupted staining. too much counterstain will mask the silver.
Created by: mustangvxd