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UND 363 PTAH
UND 363 PTAH for muscle
| Question | Answer |
|---|---|
| purpose for phosphotungstic acid hematos (PTAH) | demo muscle cross-striation (ie rhabdoyosarcomas), fibrin, nemaline rods, and glial fibers (in QC section as well) |
| principle for phosphotungstic acid hematox (PTAH) | tungsten binds hematein to blue lake (stains selected tissue blue), phospotung. acid stains others red-brown, |
| PTAH is what _______chrome stain | polychrome. 1 solution gives 2 major colors |
| what will occur if dehydration after staining is not rapid | components colored red-brown will lose color with water or prolonged alcohol washes |
| give another way to describel the principle for PTAH | phosphotungstic acid reacting with hematein created a highly acidic soln' which contains both blue and red anions (the resulting mis will stain certain tissues 2 contrasting colors) |
| fix for phosphotungstic acid hematos (PTAH) | zenkers preferred, but 10%NBF is ok |
| tech | *******4-6******** |
| qc for phosphotungstic acid hematos (PTAH) | skeletal or ciadiac muscle to demo cross-striations, or cerbral cortex for GLIAL fibers |
| results | cross-striations - blue, nuclei - blue, collagen - red-brown |
| why is zenkers used | zenkers (+acetic acid) acts as mordant if tissue was (is likely) formalin fixed |
| what needs to be used since zenkers will be in this stain | grams iodine (or lugol) and sodiom thiosulfate will remove the mercury pigment. iodine to remove mercury and sodium thio to remove excess iodine |
| what is the purpose of the pot. permanganate in this stain | dye traping agent |
| what does the oxalic acid do for this stain | decolorizes |
| how is PTAH ripened | can be done both naturally or chemically (via pot. permanganate) |
| give the order of the reagents in this stain | zenkers, gram iodine, soduim thiosulfate, pot. permanganate, oxalic acid, PTAH |
| why is the ripening of this stain important | chemically oxidized have shorter shelf life and cause overoxidation. if this happens it can affect the proper density of blue tones |
| what can be done to increase longevity of PTAH shelf life | store in amber glass bottles |
| what can also be used as a mordant for this stain | bouins |
| why is washing important in this stain | wash before staining because hydration will help with uptake of dye molecules, also washing after the sodium thiosulfate will keep it from interfering with the PTAH stain. |
| if the tissue is originally fixed in formalin what can occur | the staining can be very uneven (therefore PTAH has been mostly replaced by IHC) |
Created by:
mustangvxd
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