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UND 363 G&Sweets

UND 363 Gordon Sweets Retic.

QuestionAnswer
purpose for Gordon and Sweets reticular stain demo reticular fibers
principle for Gordon and Sweets reticular stain 1st tissue oxidized by pot. perm., oxalic acid removed excess pot. perm., ferric ammon. sensitizes and then replaced by silver, formalin makes silver visible, excess silver removed w/ sod. thio., gold toned, counterstained
what is the role of the potassium permanganate in this stain oxidizer and enhances subsequent steps
what does the oxalic acid do is this stain removes excess potassium permangenate
what is the role of ferric ammonium sulfate sensitizer (will be replaced by silver diamine during impregnation step)
Formalin is used for what reducer- makes silver visible
sodium thiosulfate is used for what removed unreacted silver
what does the gold chloride do is the toner. binds to the silver in the tissue and changed the color of the impregnated component from brown to black
nuclear fast red is used for counterstaining
fix for Gordon and Sweets reticular stain 10% NBF
tech for Gordon and Sweets reticular stain 4-5 microns
QC for Gordon and Sweets reticular stain liver
results for for Gordon and Sweets reticular stain reticulin - black, other tissue - pink to rose red if nuclear fast red used
if nuclear fast red is used as counter what must be done wash slides well in water. if not or put directly to alcohol they will develop cloudiness that can only be removed by backing up to water
why must a hint of turbidity remain in silver soln' an excess of ammonia decreased the sensitivity and results in incomplete inpregnation of reticular fibers
why is the rinse between diamine silver soln and formaldehyde important is critical for good reticulin demonstration. if wash is prolonged the reticullin staining will be reduced, if too little then excessive background stain will occur
what will help avoid unwanted (non selective precipitate) glassware chem. cleaned with commercian cleaning agent or bleach, use plastic or coated forceps
if reticular fiber pattern is important (ie liver bx) what should be seen and what can obscure it should be easy to see with scanning lens of microscope, the counterstain may obscure easy visualization and should either not be used or be light counter if visualization is very important
if nuclei are being silver stained what can be done use acetified potassium permanganate
what can be an issue with ammonium hydroxide will lose strength due to ammonia loss. Is usually noticed when increased amounts are needed to get normal results (use fresh to fix issue)
how should the deposition of silver be seen should be in a linear pattern, if it is in a granular pattern the process should be repeated
what are some hazards to ammoniacal silver can form explosive compounds. store in fridge inhibits, and avoid direct sunlight.
what is a drawback to G&S stain gives less background and nuclear staining than most other common methods
what is a drawback to silver stains in general they are moody (unpredictable), when a poor stain shows up it should be repeated with fresh stain (make sure silver diamine is slightly cloudy)
Created by: mustangvxd
 

 



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