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UND 363 Gomori retic

UND 363 Gomori reticular stain

QuestionAnswer
purpose for gomori retic. fiber stain demonstrate reticular fibers
principle for gomori retic. fiber stain hexose sugars of reticulin oxidised to aldehydes by pot. permang., excess pot perman. is removed by pot. metabisulfate, ferric ammonium sensitizes and is replaced by silver, formalin reduces to visible, toned with gold, xtra silver removed w/sod. thio.
fix for gomori retic. fiber stain 10% NBF preferred
tech for gomori retic. fiber stain 4-5 microns
qc for gomori retic. fiber stain liver used (very good control)
list the 8 reagents pot. perm - oxidizer, pot. metabisulfate -diff'r and removal of excess pot. perm., ferric ammonium - sensitizer, ammoniacal silver - impregnation, formalin - reducer, gold chloride - toner, soduim thio - fix/remove xtra silver, nuke fast red - countrstain
if liver used as spec does a counterstain need to be used No
results for gomori retic. fiber stain reticulin - black, collagen - taupe, other tissue - depends on counterstain used
why must a hint of turbidity remain in silver soln' an excess of ammonia decreased the sensitivity and results in incomplete inpregnation of reticular fibers
why is the rinse between diamine silver soln and formaldehyde important is critical for good reticulin demonstration. if wash is prolonged the reticullin staining will be reduced, if too little then excessive background stain will occur
what will help avoid unwanted (non selective precipitate) glassware chem. cleaned with commercian cleaning agent or bleach, use plastic or coated forceps
if nuclear fast red used as counterstain what must be done wash well with water after stain. if not washed well or xferred directly to alcohol they will develop cloudiness (must be backed up to water and repeated)
if reticular fiber pattern is important (ie liver bx) what should be seen and what can obscure it should be easy to see with scanning lens of microscope, the counterstain may obscure easy visualization and should either not be used or be light counter if visualization is very important
if nuclei are being silver stained what can be done use acetified potassium permanganate
what can be an issue with ammonium hydroxide will lose strength due to ammonia loss. Is usually noticed when increased amounts are needed to get normal results (use fresh to fix issue)
how should the deposition of silver be seen should be in a linear pattern, if it is in a granular pattern the process should be repeated
what are some hazards to ammoniacal silver can form explosive compounds. store in fridge inhibits, and avoid direct sunlight.
what is a drawback to silver stains in general they are moody (unpredictable), when a poor stain shows up it should be repeated with fresh stain (make sure silver diamine is slightly cloudy)
Created by: mustangvxd
 

 



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