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UND 363 Ald. Fuch.
UND 363 Aldehyde Fuchsin
| Question | Answer |
|---|---|
| Purpose for Aldehyde Fuschin elastic | demon. elastic fibers, mast cell granules, gastric chief cells, pancreatic islet Beta cells., some basophils of ant. pituitary and some mucin |
| Principle for Aldehyde Fuschin elastic | HCL and paraldehyde are added to alcholic soln' of basic fuschin (forms aldehyde fuschin), Schiff bases are formed by the aldehyde and fuchsin |
| secondary principle explaination for Aldehyde Fuschin elastic | affinity for elastic by aldehyde fuchsin is due to van der wall forces (caused btween non-polar molecules forming dipole) interacting between schiff bases and hydrophobic protein elastin |
| Fix for Aldehyde Fuschin elastic | 10% NBF preferrred, Chromate fix AVOIDED (cause cloudy staining) |
| what will formalin and bouin fixed tissue show, and if it is mercury fixed? | a colorless background for boins, pale lilac background for mercury |
| tech for Aldehyde Fuschin elastic | 4-5 micron |
| QC for Aldehyde Fuschin elastic | aorta embedded on edge or x-section of musculary artery, or can used skin |
| reagents for Aldehyde Fuschin elastic | aldehyde fuchsin (hcl/paraldehyde/alc. basic fuchsin), schiff bases - affinity for elastic fibers, alcohol - diff'r, lt. green - counterstain |
| how does the paraldehyde function | increases depth of stain by linking basic fuchsin and forming aldehyde fuchsin |
| what do schiff bases have affinity for and what color will they stain them | affinity for elastic fibers - deep purple |
| if the aldehyde fuschin is red instead of purple what is the issue | old paraldehyd used (either use fresh batch or double the conc. of the reactant) |
| if the soln' is greenish blue or purple precipitate is seen when soln' filtered what is the cause | old aldehyde fuchsin (make new solution) |
| if elastic fibers, basophils, pancreatic Beta cells not staining the basic fuchsin what may be the cause | the basic fuchsin contains 1○ rosanilin (CI42510) instead of the needed (CI42500) |
| results for Aldehyde Fuschin elastic | elastic fibers - deep blue to purple, other tissue - greeen |
Created by:
mustangvxd
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