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UND 363 Verhof Elast

UND 363 Verhoeff Elastic

Purpose for verhoeff elastic demonstrate elastic fibers and demo. atrophy of elastic tissue
principle for verhoeff elastic overstained w/ regressive heme, diff'd by excess mordant, sodium thiosulfate removes excess iodine, then counterstained with van geison or "other soln'" **elastic tissue has affinity for iron heme and will retain dye longer than other elements**
what makes up the regressive hematox iron hematoxylin/ferric chloride/iodine
what mordant diff's the stain ferric chloride breaks tissue-mordant dye complex (ie dye is attracted to excess mordant and is removed)
for verhoeff elastic principle what part does the elastic tissue have has an affinity for iron hematox and will retain dye longer than other elements (ie overstained and then diff'd back)
fix for verhoeff elastic 10% NBF or zenkers
microns for 4-5 microns
Quality control artery in CROSS SECTION and embedded ON EDGE
Reagents for verhoeff elastic Verhoeff's hematox overstain (lake of hemeatox, ferric chloride, iodine) - stain nuclei and elastic, Ferric chloride/lugols iodine (1st a mordant, 2nd oxidize hematox to hemetein, van geison - counterstain, soduim thiosulfate - removes excessive iodine
typical results for verhoeff elastic Elastic - blue/black to black, Nuclei blue to black, Collagen - Red, Other tissues - yellow
issues with over diff'ing easily done, if over diff'd restain (but can only be done if NOT treated with alcohol)
what will occur if staining prolonged in van geison the picric acid will diff the stain further
why is saturated picric acid a need collagen will not stain red and cyto/muscle/collage, will stain the same color
how must the elastic stain be prepared the reagents must be added in order and mixed after each addition or the staining may be poor
stain jar for verhoeff elastic can be cleaned how transfer the 2% ferric chloride to jar for a few min. and discard
what is a helpful hint for getting proper diff's use duplicate sections diff'd to slightly different end point
since the staining is dependant on amt. of elastic tissue what is the optimal method slides should be individually diff'd
if mercury deposits are seen what should be done nothing, they will be removed with the staining solution.
Created by: mustangvxd



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