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UND 363 VVGeison
UND 363 Verhoeff Van Geison
| Question | Answer |
|---|---|
| purpose for Verhoeff V. Geison P.A/A.F stain | primary connective tissue stain, but typically used as a counterstain for Verhoeff elastic stain |
| principle for Verhoeff V. Geison P.A/A.F stain | (3 dyes total) - collagen is selectively stained by acid dyes (ie mixture of acid dyes that compoete for available linkages) |
| what are the three stains for Verhoeff V. Geison P.A/A.F stain | Acid fuschin (sirius red can be used instead), Picric acid, Hematox (weigerts or iron celestian blue) *metal complex cationic dyes* |
| describe purpose of acid fuschin | larger molecule size permeates collagen fibers (is restricted to collagen because of its size) |
| describe purspose of Picric Acid | Smaller molecule, penetrates compact muscle, cyto, and RBC (acts as a dye itself and lowers PH of soln' for selective staining. |
| Fix for Verhoeff V. Geison P.A/A.F stain | 10% NBF |
| tech for Verhoeff V. Geison P.A/A.F stain | 4-5 microns |
| control for Verhoeff V. Geison P.A/A.F stain | built in, but can use uterus, sm. int., appenx, fall tube |
| name the three reagents for Verhoeff V. Geison P.A/A.F stain | Weigerts - stains nuclei, acid fuschin - stain collagen, picric acid - stain muscle and cyto (*must be saturated*, lowers ph and acts as counterstain) |
| what will occur if picric acid is not saturated in this stain | the collagen fibers will appear pale pink/pale orange in muscle and cyto. May stain same color as conn. tissue or appear unstained |
| what can be used in addition to van geison to help color diff. | HCL will sharpen stain diff between muscle and collagen |
| typical results for Verhoeff V. Geison P.A/A.F stain | Collagen - red, Muscle - deep yellow, Nuclei - black |
| what is the cause for lack of crispness of stain | ph is too high |
| what can cause uneven staining? | not acidic enough of stain |
| if nuclei fail to stain or are pale what is issue | likely Iron hematox was not used or it needs to be changed |
| what can cause increased red and yellow sections? | coagulent fixative will make red tones, cross linking fixatives wil cause yellow tones |
| what will occur if water is used post van geison | acid fuschin will be extracted |
| what part of the stain will direct sunlight affect | acid fuschin |
Created by:
mustangvxd
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