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UND 363 Thio T

UND 363 Thioflavin T

QuestionAnswer
purpose of Thioflavin T flourescent method demonstrate Amyloid (not as specific as congo red)
principle of Thioflavin T Thioflavin T attaches to amyloid, alum hematox will reduce the background/nuclear flourescence
that will be seen with Thio T Good visualization of even minimal deposits, but other components can be shown (light up) as well (keratin, paneth cells)
fixatives of Thioflavin T 10% NBF
thickness/control 6-10 microns, control must have known positive for amyloid
microscope for of Thioflavin T flourescent
name the 3 major reagents of Thioflavin T Mayer hematox - reduce nuclear flourescence thioflavin t - flourescent amyloid stain acetic acid - differentiation step
what type of mounting media must be avoid and give an example must avoid non flourescent mount media canada balsam media will autoflouresce
how can the thio T stain be mounted directly from water it can be mounted from water if the apathy mounting media is used
what will occur to thioflavin t in sunlight thioflavin T will detiorate quickly if kept in sunlight **also slides held for long time will deteriorate if held for long time**
typical results of Thioflavin T amyloid - flouresces yellow to yellow green
what ph is recommended for Thio T PH 1.4
What can be used to give a brighter yellow flouresce using an exciter filter UG1 and colorless UV barrier filter
why is ph important for thioflavin T it will increase selectivity of the dye for amyloid
Created by: mustangvxd
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