Busy. Please wait.
or

show password
Forgot Password?

Don't have an account?  Sign up 
or

Username is available taken
show password

why


Make sure to remember your password. If you forget it there is no way for StudyStack to send you a reset link. You would need to create a new account.
We do not share your email address with others. It is only used to allow you to reset your password. For details read our Privacy Policy and Terms of Service.


Already a StudyStack user? Log In

Reset Password
Enter the associated with your account, and we'll email you a link to reset your password.
Don't know
Know
remaining cards
Save
0:01
To flip the current card, click it or press the Spacebar key.  To move the current card to one of the three colored boxes, click on the box.  You may also press the UP ARROW key to move the card to the "Know" box, the DOWN ARROW key to move the card to the "Don't know" box, or the RIGHT ARROW key to move the card to the Remaining box.  You may also click on the card displayed in any of the three boxes to bring that card back to the center.

Pass complete!

"Know" box contains:
Time elapsed:
Retries:
restart all cards
share
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

  Normal Size     Small Size show me how

UND 363 Thio T

UND 363 Thioflavin T

QuestionAnswer
purpose of Thioflavin T flourescent method demonstrate Amyloid (not as specific as congo red)
principle of Thioflavin T Thioflavin T attaches to amyloid, alum hematox will reduce the background/nuclear flourescence
that will be seen with Thio T Good visualization of even minimal deposits, but other components can be shown (light up) as well (keratin, paneth cells)
fixatives of Thioflavin T 10% NBF
thickness/control 6-10 microns, control must have known positive for amyloid
microscope for of Thioflavin T flourescent
name the 3 major reagents of Thioflavin T Mayer hematox - reduce nuclear flourescence thioflavin t - flourescent amyloid stain acetic acid - differentiation step
what type of mounting media must be avoid and give an example must avoid non flourescent mount media canada balsam media will autoflouresce
how can the thio T stain be mounted directly from water it can be mounted from water if the apathy mounting media is used
what will occur to thioflavin t in sunlight thioflavin T will detiorate quickly if kept in sunlight **also slides held for long time will deteriorate if held for long time**
typical results of Thioflavin T amyloid - flouresces yellow to yellow green
what ph is recommended for Thio T PH 1.4
What can be used to give a brighter yellow flouresce using an exciter filter UG1 and colorless UV barrier filter
why is ph important for thioflavin T it will increase selectivity of the dye for amyloid
Created by: mustangvxd