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UND 363 Crystl Viole

UND 363 Crystal Violet

QuestionAnswer
Purpose of Crystal Violet demonstrate amyloid (not a specific as congo red)
principle of Crystal Violet *not clearly understood* polychromatic (ie single dye that stains components different colors)
the addition of acid to Crystal Violet will prevent what overstaining of the cytoplasm
fixatives of Crystal Violet 10% NBF or alcohol
thickness and control 10-12 micron, control must be a known positive for amyloid
microscope of Crystal Violet light
name the 2 reagents for crystal violet crystal violet - stains amyloid apathy mounting media
why is apathy mount media needed there is potential bleeding of basic aniline dyes into surrounding mouting medium when aqueous media is used
what does apathy mount have that prevents bleeding sugar and salt
what can be used instead of apathy and how synthetic resins can be used but stained sections must be completely air dried and then dipped in xylene before coverslipping
typical results of Crystal Violet amyloid - purplish violet other tissue - blue
Created by: mustangvxd
 

 



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