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UND 363 A.B PASh

UND 363 Alcian Blue PAS Hematox

QuestionAnswer
purpose of A.B. PAS Hematox differientate between neutral and acidic mucosubstances
Principle of A.B. PAS Hematox acidic mucosubstances stain with alcian blue while neutral mucosubstances stain with PAS
Fixatives for A.B. PAS Hematox 10% NBF or Zenkers
Thickness technique routine tissue at 4-5 microns kidney at 2-3 microns
Control slides for A.B. PAS Hematox use kidney or mucin control, using either depends on the diagnostic tissue that will be stained (can also use endo/ectocervix)
Microscope for A.B. PAS Hematox Light
name the 5 major reagents for A.B. PAS Hematox acetic acid 3% , alcian blue, periodic acid *0.5%*, schiff, harris hematox
what does the acetic acid and alcian blue do in the A.B. PAS Hematox acetic acid 3% protects the ph, alcian blue will stain the acidic mucosubstances
what does the Periodic Acid and schiff reagent do in A.B. PAS Hematox Periodic acid 0.5% - oxidizes glycols to aldehydes, Schiff reagent will stain the neutral mucosubstances
what is the purpose of the harris hematox in A.B. PAS Hematox and what issue may arise and what can fix it. acts as counterstain, easy to overstain (1 min. per book may be too long) **if overstained use rapid rinse of 0.1HCL/70%ethanol mixture
what are the expected results of A.B. PAS Hematox EXCUSIVELY acid mucosubstances - blue, neutral poly saccharides - magenta
Created by: mustangvxd