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UND 363 A.B PASh
UND 363 Alcian Blue PAS Hematox
Question | Answer |
---|---|
purpose of A.B. PAS Hematox | differientate between neutral and acidic mucosubstances |
Principle of A.B. PAS Hematox | acidic mucosubstances stain with alcian blue while neutral mucosubstances stain with PAS |
Fixatives for A.B. PAS Hematox | 10% NBF or Zenkers |
Thickness technique | routine tissue at 4-5 microns kidney at 2-3 microns |
Control slides for A.B. PAS Hematox | use kidney or mucin control, using either depends on the diagnostic tissue that will be stained (can also use endo/ectocervix) |
Microscope for A.B. PAS Hematox | Light |
name the 5 major reagents for A.B. PAS Hematox | acetic acid 3% , alcian blue, periodic acid *0.5%*, schiff, harris hematox |
what does the acetic acid and alcian blue do in the A.B. PAS Hematox | acetic acid 3% protects the ph, alcian blue will stain the acidic mucosubstances |
what does the Periodic Acid and schiff reagent do in A.B. PAS Hematox | Periodic acid 0.5% - oxidizes glycols to aldehydes, Schiff reagent will stain the neutral mucosubstances |
what is the purpose of the harris hematox in A.B. PAS Hematox and what issue may arise and what can fix it. | acts as counterstain, easy to overstain (1 min. per book may be too long) **if overstained use rapid rinse of 0.1HCL/70%ethanol mixture |
what are the expected results of A.B. PAS Hematox | EXCUSIVELY acid mucosubstances - blue, neutral poly saccharides - magenta |