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UND 363 alc. blu 1.0

UND 363 Alcian Blue 1.0

What gives the blue color of alcian blue copper
Is alcian Blue an acidic or basic dye basic dye
At 2.5 ph what are stained with alcian blue carboxylated and sulfated acid mucosubstances
At PH 1.0 what are stained with alcian blue ONLY sulfated acid mucosubstances are stained
What is used in conjunction with alcian blue to differientated epithelial and connective mucin hyaluronic acid (which preceeds the alcian blue staining in the process)
what mucin will alcian blue not stain NEUTRAL mucins
Purpose of AB 1.0 Demonstrate sulfated/sulfanated mucosubstances
principle of AB 1.0 uses 1.0 N HCL to stain ONLY sulfated acid mucopolysaccharides and sulfated sialomucins (glycoproteins)
fixatives for AB 1.0 10% NBF or Bouins
thickness and control 4-5micron, sm. intestine, appendix, or colon
microscope for AB 1.0 Light
Name the 3 major reagents with AB 1.0 0.1 N HCL, 1% alcian blue ** are combined** (stain mucin), nuclear fast red (counterstain)
What are the expected results for AB 1.0 sulfated mucosubstances - pale blue background/nuclei will be - pink to red
Created by: mustangvxd



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