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UND 363 Best Carmine

QuestionAnswer
Best Carmine Purpose Demonstrate Glycogen (though PASD is a better option)
Principle of B. Carmine Hydrogen bonds form between glycogen and carminic acids (ie hydroxyl *OH* groups of glycogen form hydrogen bonds w/ hydrogen atoms of carminic acid)
Fixatives for B. Carmine Absolute alcohol (BEST), Carnoys, Bouins
section thickness and control option sections at 4-5microns, Liver for control (DO NOT USE AUTOPSY *will likely be depleted of glycogen*
Microscope for B. Carmine Light
Name the 3 reagents used in B Carmine Carmine, harris hematox, and a differentiating solution
what is the order of the stains in B. Carmine Harris (first) as a nuclear stain, then Carmine (second) as a normal stain, lastly put in differentiating solution for the glycogen
what are the three components of the differentiating solution for B. Carmine Absolute, Methanol, distilled H20
What results should be expected with B. Carmine Glycogen = rose pink Nuclei = blue
B. Carmine contains ammonia why is this significant and what can be done ammonia is prone to evaporate and leave precipitate of dye on slide ** filter solution before using and keep in closed container** It is also a sever eye irritatnt and corrosive and should be USED under a HOOD
Created by: mustangvxd
 

 



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