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UND 363 PASD

QuestionAnswer
what does PASD demonstrate Glycogen
What is the principle behind PASD Diastase (an enzyme) depolymerizes glycogen into small sugars that are washed out. After glycogen digestion the sections are stained with PAS
What two things can be used to digest the glycogen diastase and human saliva
what fixatives are used for PASD 10 NBF, formalin alcohol, and absolute alcohol
what fixatives are least desirable for pasd picric acid, gluteraldehyde, and osmium tetroxide (they make tissue more resistant to diastase digestion therfore increasing time)
what is the proper microscope for pasd light
what is the preferred thichness 2 slides with 2 identical sections @ 4-5 microns (liver) one labeled with (digestion) and one W/O
what are good controls for pasd liver and cervix (endo and ecto cervix) the ecto contains squamous epithelium and the endo has glandular epithelium
what will the 2 epitheliums of cervix stain with the pasd reaction glandular (endo) will stain positive with schiff, while diastase will remove glycogen from squamous (ecto) therefore negative
what are the major reagents for pasd (6) malt diastase, periodic acid, schiff, metabisulfate, running water, harris hematox
what is the purpose of the malt diastase in pasd it is for glycogen digestion *contains alpha and beta enzyme*
what are some problems with malt diastase in pasd tends to loosen sections and doesn't alway complete glycogen digestion
what are some possible errors when working with malt diastase if heat beyond 40○C the digestion will cease, but if not enough heat the enzymes will not work well
based on PAS what does the P.A., Schiff, metabisulfate, water and harris do P.A. oxidises glycols to aldehydes, schiff combines with aldehydes, metabisulfate removes excess schiff, water restors schiff color, harris is used for counterstain
Created by: mustangvxd