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| Question | Answer |
|---|---|
| Selective for Salmonella; Differential for lactose | Brilliant Green |
| Inhibits most enteric gram negative bacteria | Brilliant Green |
| Brilliant Green Lactose Positive Color (decr. pH) | yellow/green/inhibited |
| Brilliant Green Lactose Negative Color | pink-white with red zone |
| Nonselective & differential for hemolysis | Sheep blood agar |
| Contains tryptone soybean protein & 5% sheep blood nutrient | Sheep blood agar |
| Supports all but the most fastidious organisms | Sheep blood agar |
| Enriched with hemoglobin, hemin (X factor), and coenzyme nicotine adenine dinucleotide (V factor) | Choco agar |
| Grows on blood agar and choco agar | N. gonorrhaeae |
| most gram pos & gram neg will grow | Sheep blood agar |
| most gram pos & gram neg will grow | Choco agar |
| Chocolate agar (only) | H. influenzae |
| Selective for gram positive bacteria; differential on the basis of hemolysis | CNA agar |
| Peptone nutrient, sheeps blood | CNA agar |
| Inhibit gram negative bacteria in CNA agar | Colistin & Nalidixic acid |
| Ability to grow staph & strep | CNA agar |
| demonstrates hemolysis (alpha & beta) | CNA agar |
| Inhibits bacteria besides Staph & Strep | CNA agar |
| Eosin Methylene Blue Agar | EMB |
| Selective for enteric gram negative rods; differentes lactose pos & neg | EMB |
| Dyes that inhibit gram positive bacteria on EMB | Eosin Y & methylene blue |
| EMB Lactose positive color | Dark purple center |
| Lactose positive occurs with: | Decrease pH |
| EMB Lactose negative color | No dark purple center |
| Lactose negative | Shigella |
| Lactose negative | Samonella |
| Lactose negative | Proteus |
| Stool specimen | Hektoen Enteric Agar |
| Very similar to Brilliant Green | Hektoen Enteric Agar |
| Selective for Salmonella, Differentiates Lactose positive & lactose negative; Differentiates H2S pos & neg | Hektoen Enteric Agar |
| Hektoen Enteric agar - dye that inhibits most normal enteric bacteria | Bromthymol blue & acid fuchsin (pH indicators) |
| H2S differential iron source | Ferric ammonium citrate |
| Hektoen Enteric L+ | Salmon/yellow colonies |
| Hektoen Enteric L- | Green,blue |
| Hektoen Enteric H2S+ | Black center in colony |
| Mac Conkey (similar) | EMB (similar) |
| Differential based on lactose; Selective for gram negative rods | Mac Conkey |
| Mac Conkey specimens | stool, sputum, urinary |
| Mac Conkey dye that inhibits gram + bacteria | crystal violet dye |
| Mac Conkey Lactose + color | Red |
| Mac Conkey Lactose - color | colorless |
| Selective for Staph; Differential due to Mannitol Salt | Mannitol Salt agar |
| Selective for Staph | Mannitol Salt agar |
| Mannitol Salt agar - what inhibits all gram - & all gram + but Staph | Na Cl |
| Differentiates wh/ Staph ferment Mannitol & wh/ don't | Mannitol |
| Ferments Mannitol color | Yellow colonies with yellow zones |
| Nonfermentor of Mannitol color | Pink |
| Phenylethyl Alcohol Agar | PEA |
| PEA (similar) | CNA (similar) |
| Selective for Staph & Strep; Differential due to hemolysis | Phenylethyl Alcohol Agar |
| Inhibits gram negative bacteria in PEA | Phenylethyl alcohol |
| Selective for Strep (Beta hemolysis) | SXT agar |
| Sheep blood agar with trimethoprim-sulfa | SXT |
| Inhibits most bacteria other than Group A & Group B strep | SXT/TMP/smx |
| Beta hemolysis | S. pyogenes & S. agalactiae |
| Stool Specimen | Selenite F Broth |
| Toxic for normal enteric flora | Sodium selenite |
| Enhances enteric pathogens - especially salmonella | Selenite broth |
| Salmonella (good growth); Shigella (partial or complete inhibition) | Selenite broth |
| Selective for N. gonorrhoeae | Thayer Martin Agar |
| Chocolate agar + antibiotics | Thayer Martin Agar |
| Thayer Martin - Inhibit other gram negative | Colistin |
| Thayer Martin - Inhibit gram positive | Vancomycin |
| Thayer Martin - Inhibit yeast | Nystatin |
| Modified Thayer Martin (MTM) | Trimethoprim - inhibits proteus |
| Normally sterile body sites | Thioglycollate broth |
| Permits growth of anaerobes | Thioglycollate broth |
| Permits growth of anaerobes | Thio, cystine (lowers oxidation) |
| Enhance the growth of pathogens that are present in small numbers | Thioglycollate broth |
| Enhance the growth of most bacteria | Beef protein, glucose |
| Xylose, Lysine - Desoxycholate Agar | XLD |
| Differential due to lactose & H2S; Selective for Salmonella & Shigella | Xylose Lysine - Desoxycholate Agar |
| XLD - Inhibits gram + bacteria & some enterics | Na desoxycholate & salts |
| XLD Lactose positive color | Yellow colonies |
| XLD Lactose negative color | Pale pink colonies |
| XLD - H2S | Ferric ammonium citrate |
| XLD - H2S + | Black center |
| XLD - Pink colonies with black center | Salmonella |
| XLD - Pink colonies with no black center | Shigella |
| Salmonella | Lactose negative |
| Salmonella | H2S positive |
| Shigella | Lactose negative |
| Shigella | H2S negative |
| E. coli | Lactose positive |
| E. coli | H2S negative |
Created by:
tswalker_3
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