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Protein Diagnostic
WVSOM Class of 2012 Protein Diagnostic Techniques
Question | Answer |
---|---|
3 methods used to separate proteins are: | size exclusion, HPLC, and ion exchange |
gel electrophoresis uses which method to separate proteins? | size exclusion |
in studying proteins, which proteins will stay at the top of the gel? | larger proteins |
what are the 3 ck markers? | ck1-brain, ck2-heart, ck3-muscle |
what is HPLC? | high phase liquid chromatography |
ion exchange separates proteins based on what? | charge |
in an antibody, there are how many light and heavy chains? how many are variable and constant? | 2 light: 2 variable, 2 constant; 2 heavy: 1 variable, 3 constant |
in order to form more AAs, what AA must you have? | cysteine |
what are the steps of a western blot? | electrophoresis and transfer, incubate with ab1 and wash excess, incubate with enzyme linked ab2 and wash excess, react with substrate for ab2 linked enzyme |
what are the steps of proteomics? | sort proteins by charge, then put that gel on another, sort proteins by size (in second dimension) |
alzheimer's disease has what specific plaque formation? | beta-amyloid |